黑曲霉产β-葡萄糖苷酶发酵条件的研究  

Study on Fermentation Condition of Aspergillus Niger Producing β-glucosidase

作　　者：赵子高[1] 吕世峰[1] 杨付伟[1] 闫振丽[1] 王林凤[1] 

机构地区：[1]河南天冠企业集团有限公司,河南南阳473000

出　　处：《山东化工》2010年第9期12-15,18,共5页Shandong Chemical Industry

基　　金：国家科技支撑计划项目(2007BAD66B01)

摘　　要：通过单因素及正交实验对现存黑曲霉菌株产酶发酵条件进行了研究,结果表明:当培养基为:玉米芯4.0%;酵母粉1.0%;KH2PO40.4%;MgSO40.08%;(NH4)2SO40.4%;培养温度30℃,摇床转速220r/min,接种量6.0%,装液量40mL/250mL,初始pH值5.0,发酵周期168h;此条件下β-葡萄糖苷酶酶活达56.96IU/mL。An Aspergillus niger producing β-glucosidase was selected. Its medium ingredients and fermentation conditions were optimized by single factor and orthogonal experiments. The results were as follows： corncob 4.0% ; yeast 1.0% ; KH2PO40.4% ; MgSO4 0.08% ; （ NH4 ）2SO4 0.4% ; culture temperature 30℃, rotation speed 220r/min, inoculation volume 6. 0% , the amount of liquid 40mL （250mL canonical flask） , initial pH 5.0 and fermentation time 168 hours. Under these conditions ,The activity of - glucosidase was reach 56.96IU/mL.

关 键 词：黑曲霉 Β-葡萄糖苷酶 培养基 酶活力 发酵条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]