纤维素酶-木聚糖酶共固定化的基础研究  

Basic studies of the co-immobilization of cellulase and xylanase

作　　者：徐子栋[1] 缪冶炼[1] 陈介余 蒋学剑[1] 钱莉[1] 林丽军[1] 

机构地区：[1]南京工业大学食品与轻工学院材料化学工程国家重点实验室 [2]日本秋田县立大学生物资源学部

出　　处：《食品科技》2010年第10期2-7,共6页Food Science and Technology

基　　金：国家自然科学基金委-广东省政府联合基金(U0733001);国家重点基础研究发展计划(973项目;2009CB724700)

摘　　要：以pH敏感型可逆可溶性聚合物EudragitL-100为载体,同时固定由里氏木霉生产的纤维素酶和木聚糖酶。首先考察了pH对酶活力的影响和载体的可逆可溶特性,然后调查了固定化过程中载体浓度和交联剂(1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐,EDC)浓度对酶活固定率的影响。实验结果表明,在4.0～6.0的pH范围内,纤维素酶的稳定性较好;添加适量的EDC可以提高载体沉淀所需的pH,同时加强酶与载体的连接;当载体浓度为2%、EDC浓度为0.1%、50mL载体溶液中纤维素酶和木聚糖酶的用量分别为50FPU和400U时,纤维素酶和木聚糖酶的酶活固定率分别达到75%和59%,3次重复固定后的酶活固定率仍保留有56%和40%。Eudragit L-100, a reversibly soluble-insoluble polymer, was used for the simultaneous immobilization of cellulase and xylanase from Trichoderma reesei. First, the effect of pH on the enzyme activity and the reversible solubility of carrier were studied. Then, the effects of carrier concentration and cross-linker （1-（3dimethylaminopropyl） -3 -ethylcarbodiimide hydrochlorid, EDC） concentration on the activity recovery of enzymes in immobilization process were investigated. It was shown that the cellulase had a stable activity in the pH range of 4.0 to 6.0. The pH for precipitation of carrier was increased by adding an appropriate amount of EDC, and the links between carriers and enzymes were strengthened accordingly. An activity recovery of 75% and 59% was obtained for cellulase and xylanase, respectively, under the condition of carrier con centration at 2%, EDC concentration at 0.1%, cellulase loading at 50 FPU and xylanase loading at 400 U in 50 mL carrier solution. After reused for 3 times, the activity recovery of cellulase and xylanse was still remained at 56% and 40%, respectively.

关 键 词：载体 交联剂 固定化 纤维素酶 木聚糖酶 

分 类 号：TS201.25[轻工技术与工程—食品科学]