气质联用法测定丁酸单甘酯  

Determination of Monobutyrin by GC-MS

作　　者：易路遥[1,2] 郑为完[1] 廖和菁[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西省食品药品检验所,江西南昌330046

出　　处：《食品科学》2010年第20期402-405,共4页Food Science

摘　　要：建立GC-MS测定丁酸单甘酯含量的方法。采用DB-5MS(30m×0.25mm,0.25μm)毛细管柱,进样口温度280℃,色谱升温条件为起始100℃,以10℃/min升至150℃,再以30℃/min升至250℃,保持5min,氦气流量1.0mL/min,分流比10:1。结果表明:丁酸单甘酯质量浓度在0.5～10μg/mL范围内呈良好线性关系,线性方程Y=146961X-30250(r=0.9997),平均加标回收率为99.9%,重复性实验RSD为1.7%,最低检测限0.2ng。该方法简便、准确且重复性好,可用于丁酸甘油酯中丁酸单甘酯的含量测定。A GC-MS method for quantitative analysis of monobutyrin was established. The determination parameters were the capillary column DB-5MS （30 × 0.25 mmm,0.25μm）,injection temperature of 280 ℃ programmed temperature increase from 100 to 150 ℃ with an increase rate of 10 ℃/min,then programmed temperature increase from 150 ℃ to final temperature of 250 ℃ at an increase rate of 30 ℃/min,helium flow rate of 1.0 mL/min and split ratio of 10：1,respectively. Results indicated that a good linear relationship between integral value （Y） of peak area and the content of monobutyrin （X） in the range of 0.5-10μg/mL was observed and the linear equation was Y=146961X-30250 （r=0.9997）. Recovery rate of monobutyrin were 99.9% with the RSD of 1.7%. The limit of determination was 0.2 ng. This method is simple,accurate and reproducible so that it can be used for the determination of monobutyrin.

关 键 词：丁酸单甘酯 气相色谱-质谱 测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]