米曲霉利用农业废弃物产木聚糖酶的条件优化  

OPTIMIZATION OF XYLANASE PRODUCTION FROM ASPERGILLUS ORYZAE BY USING AGRICULTURAL WASTES IN SUBMERGED FERMENTATION

作　　者：李勇如[1] 李秀婷[1] 孙宝国[1] 宋焕禄[1] 吕跃钢[1] 宋红霞[1] 

机构地区：[1]北京工商大学化学与环境工程学院/食品添加剂与配料北京市高校工程研究中心,北京100048

出　　处：《北京工商大学学报（自然科学版）》2010年第5期18-23,共6页Journal of Beijing Technology and Business University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31071511);北京市属高等学校人才强教计划资助项目(PHR20110872);北京工商大学教改项目(jg095206)

摘　　要：主要研究了米曲霉利用农业废弃物产木聚糖酶的情况.对实验室保藏菌株米曲霉M-9生长所必需的碳源、氮源、培养温度和培养基初始pH等条件进行了优化.实验表明,此菌株最适碳源是粒度小于0.08 mm的玉米芯(质量分数为4.0%),最佳复合氮源是质量分数为1.2%的酵母提取物加质量分数为0.8%的大豆蛋白胨,最适初始pH值为7.5,最适产酶温度为30℃,最佳转速为200 r/m in,添加表面活性剂吐温60对菌株产酶有促进作用.在最适培养条件下,培养5 d,米曲霉沪酿M-9木聚糖酶的产量高达795.93 U/mL,是优化前的2.2倍.Cultural conditions for xylanase production by the strain Aspergillus oryzae M-9 were optimized under submerged fermentation.The strain secretes high levels of xylanase on the agricultural residues of Corn cobs as well as on xylans.The results of single-factor experiment showed that 0.08mm Corn cobs（4.0%） was the best carbon source and inducer.A combination of yeast extract（1.2%） and soybean peptone（0.8%） was the best nitrogen source.The optimal initial pH value,cultivation temperature and the rotation rate of the cultures were pH 7.5,30℃ and 200r/min,respectively.The maximum increase in xylanase production was observed in the surfactants of Tween 60.Under the optimized conditions,the highest xylanase activity of 795.93U/mL was obtained in the 5th day.

关 键 词：米曲霉 木聚糖酶 发酵 农业废弃物 

分 类 号：TS201.3[轻工技术与工程—食品科学]