分批与流加培养对萼状粒毛盘菌生物量和胞外多糖产量的影响  

Effects of batch and fed-batch cultures on biomass and exopolysaccharide production of Lachnum calyculiforme

作　　者：叶明[1] 陈吴西[1] 彭伟[1] 马健[1] 杨柳[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《浙江大学学报（农业与生命科学版）》2010年第6期630-634,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(31070021);安徽省高校自然基金重大资助项目(kj2010zd10)

摘　　要：通过摇瓶实验对萼状粒毛盘菌(Lachnumcalyculiforme)生物量及其产胞外多糖的发酵条件进行研究;并在5L发酵罐中进行分批与流加培养,比较二者对萼状粒毛盘菌生物量及其多糖产量的影响.结果表明:在25℃时,粒毛盘菌生物量和产胞外多糖的最佳碳、氮源及其浓度为葡萄糖30g L-1、酵母膏5g L-1,pH值为6.0;10g L-1橄榄油能显著加速菌丝体的生长,而10g L-1大豆油能明显提高胞外多糖的产量;分批培养时粒毛盘菌的最大生物量和多糖产量分别为6.40和8.28g L-1,而在葡萄糖浓度低于5g L-1时进行流加培养可以显著提高粒毛盘菌的生物量和多糖产量,最大生物量和多糖产量分别为7.72和11.82g L-1.The effects of cultural conditions on the biomass and exopolysaccharide（EPS）production of Lachnum calyculiforme were studied by shake flask experiments.Batch and fed-batch cultures were held in 5 L stirred fermentor,and the effects of the two culture methods on the biomass and EPS production of L.calyculiforme were compared.At 25 ℃,shake flask experiments indicated that the optimum conditions for biomass and EPS production of L.calyculiforme were as follows：glucose 30 g·L-1,yeast extract 5 g·L-1 and the pH 6.0.The 10 g·L-1 olive oil significantly stimulated the mycelial growth,and 10 g·L-1 soybean oil could enhance EPS production.The highest biomass and EPS production of L.calyculiforme in the batch fermentation were 6.40 and 8.28 g·L-1 respectively,but those of the fed-batch fermentation by glucose feeding were 7.72 and 11.82 g·L-1 respectively when the glucose concentration in the medium was lower than 5 g·L-1,showing that feeding glucose can enhance greatly the accumulation of biomass and EPS of L.calyculiforme.

关 键 词：胞外多糖 分批培养 流加培养 萼状粒毛盘菌 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]