天祝牧区牦牛乳酥油中产脂肪酶菌株筛选及产酶条件的研究  

Screening lipase-producing microorganisms from yak butter of Tianzhu pasturing area and study on its fermentation conditions

作　　者：孙国政[1,2] 甘伯中[1,2,3] 张卫兵[1,2] 李晓鹏[1,2] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]甘肃农业大学研究测试中心,甘肃兰州730070 [3]甘肃省干酪素工程技术研究中心,甘肃兰州730000

出　　处：《食品工业科技》2010年第12期195-199,共5页Science and Technology of Food Industry

基　　金：甘肃省科技重大专项(0702NKDA034)

摘　　要：从甘肃省天祝牧区牦牛乳酥油中分离筛选产脂肪酶微生物。经初筛、复筛后得菌株S122产脂肪酶活力较高;形态学观察和26SrDNA序列比对分析表明该菌为Geotrichum sp·S122。对菌株Geotrichum sp·S122发酵产酶条件进行研究表明,其最适的发酵培养基组成成分(%):蔗糖1·0、蛋白胨2·0、硫酸镁0·1、磷酸氢二钾1·0、橄榄油乳化液1·0;最适发酵产酶条件:培养基初始pH8·0,培养温度28℃,接种量4%,装液量60mL/250mL,摇床转速200r/min,发酵周期96h,优化后脂肪酶活力最高达59U/mL。Lipase-producing microorganisms were isolated from yak butter in the Tianzhu pasturing area of Gansu province.The high lipase-producing strain S122 was isolated by secondary screening.It was identified as Geotrichum sp.S122 through the morphological feature observation and 26S rDNA sequence analysis.The fermentation conditions of the lipase-production strain were optimized and fermentation techniques on the lipase production were studied.The results showed that the optimal medium composition for lipase production was as follows（%）：sucrose 1.0,peptone 2.0,MgSO4 0.1,K2HPO4 1.0,olive oil 1.0.The optimal fermentation conditions were as follows：the initiation pH 8.0 for the culture medium,the optimal culture temperature 28℃,inoculation volume 4%,rotation speed 200r/min,culture medium 60mL/250mL（v/v）and the culture time 96h.After optimization,the lipase activity was 59U/mL.

关 键 词：牦牛乳酥油 脂肪酶 分离筛选 产酶条件 

分 类 号：TS201.1[轻工技术与工程—食品科学]