超声波对酶法水解丝素作用的研究  

Study on the effect of ultrasound on fibroin hydrolyzed by protein enzymes

作　　者：李利军[1] 吴启涛[1] 杨峰[1] 黄永春[1] 李彦青[1] 赵小进[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《食品工业科技》2010年第12期276-278,281,共4页Science and Technology of Food Industry

摘　　要：以废蚕丝的再生丝素液为原料,研究了有无超声作用、底物浓度、时间、温度、pH、加酶量等因素对碱性蛋白酶水解制备丝素肽的影响。结果表明,超声辅助酶解再生丝素液制备丝素肽最佳工艺为:底物浓度4·0%、酶浓度为4·0%、反应温度为45℃、反应时间为40min、pH为10·0、超声功率为200W。与水浴酶解相比,超声作用没有改变回收率与水解温度、pH、底物浓度、加酶量等因素的变化关系;但超声作用可提高回收率,缩短反应时间。Taking regenerated solution of waste silk fibroin as material,the effects of the application of ultrasound,reactant concentration,time,temperature,pH and enzyme concentration on silk peptide hydrolyzed by alkaline protein enzyme and silk amino acid mixture were investigated by using single factor experiment.The optimal parameters were：reactant concentration 4.0%,enzyme concentration 4.0%,extraction temperature 45℃,reaction time 40min,pH 10.0,ultrasound power 200W.Compared with water bath hydrolyzing,ultrasound-assisted reaction is more effective with advantages in short reaction period and improving response rate.Though it cannot change the relationship among the recovery rate and reactant or enzyme concentration,temperature,pH,it can increase recovery rate and shorten time.

关 键 词：废蚕丝素 超声 酶解 丝素肽 

分 类 号：TS209[轻工技术与工程—食品科学]