薄层层析-生物自显影法筛选油菜蜂花粉中活性成分  

Using TLC-bioautography to Screen Active Compounds in Rape Bee Pollen

作　　者：孙丽萍[1,2] 杜夏[1] 徐响[1,2] 董捷[1,2] 安仲姚[1] 杨佳林[1] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]国家农产品加工技术研发中心蜂产品分中心,北京100093

出　　处：《食品科学》2010年第21期69-71,共3页Food Science

基　　金：农业部公益性行业(农业)科研专项(nyhyzx07-041);国家现代农业(蜂)产业技术体系建设专项(NYCYTX-43);农业科技成果转化资金项目(2009GB23260456)

摘　　要：采用薄层层析-生物自显影法筛选油菜蜂花粉中清除DPPH自由基的活性成分和抑菌活性成分。结果显示:油菜蜂花粉中清除DPPH自由基的黄酮类活性成分主要以苷的形式存在,酸解后生成了包括槲皮素和山奈酚在内的多种抗氧化成分;在Rf值0.29和0.86处存在抑菌活性成分,不同显色方法确认,抑菌活性成分的种类可能包括酚类、鞣质、黄酮类或皂苷类。TLC-bioautography was used to screen the DPPH radical scavengers and the antibacterial ingredients in rape bee pollen.The experimental results indicated the flavonoids as DPPH radical scavengers mainly existed in form of glycosides in rape bee pollen,and the acidicly hydrolyzed ethanol extract from rape bee pollen contained a variety of antioxidant compounds including quercetin and kaempferol.There were two small molecules with Rf values of 0.29 and 0.86 found to have antibacterial activity in the water extract from rape bee pollen.According to the results of color development reaction,the types of antibacterial components were polyphenols,tannin,flavonoids or saponins,etc.

关 键 词：油菜蜂花粉 薄层层析-生物自显影 抗氧化活性 抑菌活性 

分 类 号：S896.4[农业科学—特种经济动物饲养]