脱脂麦胚中谷胱甘肽与麦胚蛋白提取  

Extraction of Glutathione and Wheat Germ Protein from Defatted Wheat Germ

作　　者：黄纪念[1] 宋国辉[1] 孙强[1] 詹传保[1] 魏红[1] 

机构地区：[1]河南省农业科学院农副产品加工研究所,河南郑州450002

出　　处：《食品科学》2010年第22期139-144,共6页Food Science

基　　金：河南省农业科学院科研发展专项

摘　　要：对脱脂麦胚中谷胱甘肽(GSH)和麦胚蛋白的提取进行研究。首先采用低pH值条件从脱脂麦胚中提取谷胱甘肽,并采用超滤将该提取液中的谷胱甘肽与蛋白质进行分离。再对提取过GSH后的麦胚残渣中的蛋白质进行碱提研究。最后合并超滤分离出的蛋白质与碱提蛋白质,采用等电点沉淀法对麦胚蛋白进行纯化制备。采用单因素试验结合正交试验分别对GSH和麦胚蛋白的提取工艺进行优化,并最终确定GSH最佳提取工艺为提取pH6.3、提取时间15min、固液比1:13(g/mL)、提取温度55℃,GSH的得率和提取率分别为0.31%和72.1%。麦胚蛋白的最佳提取工艺为提取pH10.5、提取温度65℃、固液比1:11、提取时间60min。提取蛋白质相对于碱提用沉淀的得率为39.2%,提取率为90.4%。相对于总麦胚得率为20.8%,提取率为58.8%。合并超滤截留液与碱提离心上清液,确定沉淀蛋白质最佳pH4.2,蛋白质沉淀率为97.5%,所得蛋白质含量达85.2%,相对于脱脂麦胚中总蛋白质提取率为81.1%。In this paper, the extraction and separation of glutathione （GSH） and wheat germ protein from defatted wheat germ was investigated. Glutathione was extracted from defatted wheat germ under the low pH condition. Glutathione and wheat germ protein in extract were separated by ultra-filtration method. The protein residue after GSH extraction was subjected to further alkali extraction. The obtained protein was purified by isoelectric precipitation method. The extraction conditions for GSH were optimized by single factor and orthogonal experiments to be material-liquid ratio of 1：13, pH of 6.3, extraction temperature of 55 ℃ and extraction time of 15 min. Under the optimal extraction conditions, the yield and extraction rate of GSH was up to 0.31% and 72.1%. The extraction conditions for wheat germ protein were optimized by single factor and orthogonal experiments to be material-liquid ratio of 1：11, pH of 10.5, extraction temperature of 65 ℃ and extraction time of 60 min. Under the optimal extraction conditions, the yield and extraction rate of protein was up to 39.2% and 90.4% based on the amount of alkali extraction precipitate. The yield and extraction rate of protein were 20.8% and 58.8% based on the amount of defatted wheat germ. The ultra-filtration liquid and supernatant after alkali precipitation was combined. The optimum pH for precipitating protein from combined solution was 4.2. The precipitation rate and yield of protein were 97.5% and 85.2%. After the combination, the extraction rate of protein was 81.1% based on the amount of deffated wheat germ.

关 键 词：脱脂麦胚 谷胱甘肽 麦胚蛋白 提取 等电点沉淀 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]