旋光法测定发酵液中2-酮基-D-葡萄糖酸  

Determination of 2-Keto-D-gluconic Acid in Fermentation Broth by Polarimetry

作　　者：孙文敬[1] 周延政[1] 冯晓燕[2] 魏转[3] 余泗莲[4] 周强[4] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]河北师范大学生命科学学院,河北石家庄050016 [3]河北化工医药职业技术学院制药工程系,河北石家庄050026 [4]百勤异VC钠有限公司,江西德兴334221

出　　处：《食品科学》2010年第22期375-377,共3页Food Science

基　　金：江苏大学科研启动基金项目(08JDG029);江西省主要学科学术和技术带头人培养计划项目(2008DD00600);江西省科技支撑计划项目(赣财教[2008]147号)

摘　　要：通过测定发酵液的旋光度及其葡萄糖质量浓度,计算发酵液中2-酮基-D-葡萄糖酸的质量浓度,并考察该方法实际应用的可行性。结果表明:对照品溶液的旋光度、2-酮基-D-葡萄糖酸质量浓度和葡萄糖质量浓度之间存在良好的线性关系,R2>0.9999;该方法精密度、稳定性和重现性良好,2-酮基-D-葡萄糖酸的平均回收率为102.60%,RSD为2.17%(n=5)。该方法可用于发酵液中2-酮基-D-葡萄糖酸的定量检测。The 2-keto-D-gluconic acid in fermentation broth was calculated on the basis of glucose concentration determined by optical rotation degree. The application feasibility of polarimetry was also investigated. Results indicated that a good linear relationship between optical rotation degree and the concentration of 2-keto-D-gluconic acid and glucose （R2 0.9999）. The average recovery rate was 102.60% with RSD of 2.17%. This method has characteristics of precision, stability and reproducibility. Therefore, it can be used for determining 2-keto-D-gluconic acid in fermentation broth.

关 键 词：2-酮基-D-葡萄糖酸 葡萄糖 发酵液 旋光法 测定 

分 类 号：TQ921.2[轻工技术与工程—发酵工程]