黑木耳多糖提取工艺条件的研究  

Optimization of extraction conditions of auricularia auricula polysaccharides

作　　者：陆雯[1] 蔡振优[1] 鲜乔[1] 张拥军[1] 

机构地区：[1]中国计量学院生命科学学院,杭州310018

出　　处：《中国食品添加剂》2010年第6期99-102,共4页China Food Additives

摘　　要：黑木耳多糖是黑木耳子实体中的重要活性物质,除具有一般的生化性质外,还具有调节免疫功能、抗血栓、抑菌、降血糖、抗溃疡、抗肝炎和抗突变、降血脂和促进血清蛋白生物合成等多种功效。为优化黑木耳多糖的提取工艺,本文在单因素试验结果的基础上,选择料液比、提取温度、提取时间为自变量,黑木耳多糖的得率为响应值,研究各自变量交互作用及其对黑木耳多糖得率的影响,确定了黑木耳多糖提取的最佳条件为:提取温度60℃、料液比为1∶40、提取时间为2.5h,在此最佳提取条件下,黑木耳多糖的得率达到5.12%。对提取的黑木耳多糖的营养成分进行了分析,所制备的多糖以大分子多糖为主,还含有一定量的蛋白质、水分、还原糖及灰分,为一种粗多糖。Auricularia auricula polysaccharide（APS）is an important bio-active material.Except with the general physicochemical and biological properties,APS possess the immunomodulatory effect,lovenox,bacteriostasis,anti-diabetics,atherosclerosis.In this study,optimum condition of extraction of APS is studied.On the basis of single-factor test,the mathematical regression model is established based on the dependent variable（extraction yield of APS）and independent variables（ratio of material to water,extraction temperature and extraction time）.The optimum extraction conditions are as follows：material to water ratio 1 ∶40,extraction temperature 60℃,time 150min.Under these conditions,the yield of APS is 5.12%.The chemical composition of APS was tested.The polysaccharides are mainly large molecules with a certain amount of protein,moisture,reducing sugar and ash.

关 键 词：黑木耳 多糖 提取 营养成分 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]