苦荞萌发物中生物活性黄酮对人乳腺癌细胞增殖的抑制作用  

Inhibitory Effect of Flavonoids from Tartary Buckwheat Sprouts on Proliferation of Human Breast Cancer Cells

作　　者：周小理[1] 王青[1,2] 杨延利[3] 李宗杰[1] 唐文[1] 

机构地区：[1]上海应用技术学院香料香精技术与工程学院,上海200235 [2]上海海洋大学食品学院,上海201306 [3]上海师范大学生命与环境科学学院,上海200234

出　　处：《食品科学》2011年第1期225-228,共4页Food Science

基　　金：国家自然科学基金项目(31071527);上海市科委重点科技攻关项目(074905114)

摘　　要：以萌发期(1～6d)的苦荞芽粉为原料,采用铝盐显色可见分光光度法和HPLC法,探明萌发期(1～6d)苦荞芽粉乙醇提取物中总黄酮、芦丁和槲皮素含量的变化趋势。以人乳腺癌细胞MCF-7为模型,采用噻唑蓝(MTT)比色法,比较萌发期(1～6d)的苦荞芽粉乙醇提取物对人乳腺癌细胞体外的增殖抑制率。结果表明:苦荞芽粉乙醇提取物具有抑制MCF-7乳腺癌细胞增殖的作用,尤以萌发第3天(芦丁与槲皮素含量比为0.92:1)时抑制效果最好,显示二者具有良好的协同抑制效果;苦荞芽粉乙醇提取物的抑制效果与槲皮素和芦丁标准品模拟样品抑制效果相似,表明苦荞芽粉乙醇提取物对MCF-7细胞的生长起抑制作用的主要功效成分为槲皮素和芦丁。The contents of total flavonoids,quercetin and rutin in the powders of tartary buckwheat sprouts germinated for 1 through 6 days were determined using spectrophotometery and HPLC.The anticancer effect of total flavonoids from tartary buckwheat sprouts on human breast cancer MCF-7 cells was evaluated by MTT assay.Results indicated that the ethanol extracts（rich in flavonoids） from all the tartary buckwheat sprouts exhibited an obvious inhibitory effect on the proliferation of MCF-7 cells,especially for the extract from tartary buckwheat sprouts germinated for 3 days with a rutin/quercetin ratio of 0.92：1.Meanwhile,a synergistic effect of rutin and quercetin on the proliferation of MCF-7 cells was observed.Moreover,a similar inhibitory effect between ethanol extracts from tartary buckwheat sprouts and the standard samples of quercetin and rutin on the proliferation of MCF-7 cells was observed.Therefore,these investigations demonstrate that the major components in tartary buckwheat sprouts contributing to the inhibitory effect on tumor are quercetin and rutin.

关 键 词：苦荞 萌发 黄酮 槲皮素 芦丁 MCF-7细胞 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]