树脂固定化α-乙酰乳酸脱羧酶的初步研究  

Preliminary study on immobilization of α-acetolactate decarboxylase by resin

作　　者：陈卿[1,2] 蔡国林[2] 陆健[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品工业科技》2011年第2期154-158,共5页Science and Technology of Food Industry

基　　金：国家"十一五"支撑重点项目资助(2008BAI63B06);国家"十一五"支撑计划项目(2007BAK36B01);江苏省"青蓝工程"资助项目

摘　　要：采用7种树脂对α-乙酰乳酸脱羧酶进行固定化,并将固定化酶应用于啤酒发酵实验,从中筛选出降低发酵液中双乙酰能力较强的大孔弱碱性丙烯酸系阴离子交换树脂D314FD作为固定化的载体。通过单因素和正交实验确定固定化ALDC的最优条件为:每克树脂加入1.25mL原酶液,吸附时间10h,吸附pH7.5,吸附温度15℃,在此条件下固定化效率可达44%。发酵实验亦表明,固定化ALDC可以显著降低发酵液中双乙酰的含量,降低率达40%以上。Alpha-acetolactate decarboxylase was immobilized on 7 kinds of resins through adsorption,and then they were used in beer maturation. Among them,weak macroporous ion-exchange resin D314FD was selected as immobilized carrier for its good ability on reducing the content of diacetyl. The results showed that the optimum conditions for α-acetolactate decarboxylase immobilized as follows：1.25mL liquid α-acetolactate decarboxylase was added into one gram of pretreated resin,stirred for 10h at 15℃ in buffer of pH7.5. Under the optimal conditions,the immobilization efficiency could reach 44%. Fermentation trial also showed that immobilized ALDC could significantly reduce the content of diacetyl,and the rate of reducing was up to 40%.

关 键 词：树脂 Α-乙酰乳酸脱羧酶 固定化 

分 类 号：TS201.25[轻工技术与工程—食品科学]