大豆蛋白酶解物的抗癌活性研究  

Research on anti-cancer activities of soybean protein hydrolysates

作　　者：吴非[1] 于胜男[1] 葛锡娟[1] 李良[1] 王鹏[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030

出　　处：《东北农业大学学报》2010年第12期98-102,共5页Journal of Northeast Agricultural University

基　　金：黑龙江省教育厅科学技术研究面上项目(11531016)

摘　　要：以大豆分离蛋白为原料,用中性蛋白酶、木瓜蛋白酶、碱性蛋白酶和胃蛋白酶分别对其进行酶解,得到具有抗癌活性的酶解物,测定大豆分离蛋白的水解度和酶解物对胃癌细胞的生长抑制率。结果表明,木瓜蛋白酶酶解物的癌细胞生长抑制率为27.36%,显著高于其他三种酶解物的抗癌活性,其次是中性蛋白酶(21.20%)和碱性蛋白酶(18.01%),酶解物抗癌活性最低的是胃蛋白酶(11.20%)。四种蛋白酶在最适水解条件下作用于大豆分离蛋白时,水解度的排列次序为:碱性蛋白酶>中性蛋白酶>木瓜蛋白酶>胃蛋白酶。大豆分离蛋白的水解度与酶解物的细胞生长抑制率不呈线性关系。Soy protein isolate was treated as raw material.The hydrolysates with anti-cancer activities were prepared by enzymolysis separately with neutral protease,papain,alkaline protease and pepsin.The hydrolysis degrees and cancer cell growth inhibitory values of hydrolysates were detected.The results showed that the anti-cancer activity of the papain hydrolysate was 27.36%,higher than those of other protease hydrolysates,remarkably.The followed ones were neutrase hydrolysate（21.20%） and Alcalase hydrolysate（18.01%）.The pepsin hydrolysate had the lowest cancer cell growth inhibitory value（11.20%）.On the optimum enzymolysis conditions,the hydrolysis degree order of the soybean protein was like this： alkalaseneutrasepapainpepsin.There was no linear relationships between cancer cell growth inhibitory values and the hydrolysis degrees.

关 键 词：大豆分离蛋白 酶解物 抗癌活性 水解度 

分 类 号：S565.1[农业科学—作物学]