Protamex复合蛋白酶酶解猪骨的工艺研究  

Processing of Enzymolysis of Compound Proteinase Named Protamex on Pork Bones

作　　者：白艳红[1] 陈茜[1] 赵电波[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,郑州450002

出　　处：《食品工业》2011年第1期88-91,共4页The Food Industry

基　　金：河南省重点科技攻关计划项目(09210211031)

摘　　要：研究并优化采用Protamex复合蛋白酶酶解猪骨的工艺条件。以酶解温度、时间、pH值、加酶量、料液比为因素,以水解度为指标,采用正交试验对酶解工艺参数进行优化。结果表明,利用Protamex复合蛋白酶酶解猪骨的最佳条件是:酶解温度45℃、pH7.0、酶解时间6 h、加酶量0.4%(W/W)、料液比1∶10(W/V)。在此条件下,水解度可达到10.08%。研究结果为工业化猪骨副产物的综合利用提供理论依据和技术支持。The process parameters of enzymolysis of compound proteinase named protamex on pork bones were studied.The orthogonal experiment design was used with which enzymolysis temperature,time,pH,additive amount of protamex,and solid-liquid ratio were as influence factors and degree of hydrolysis（DH） was as index value.The results showed that 10.08% DH could be obtained when the temperature was of 45℃,pH was of 7.0,time was of 6h,the additive amount of protamex was of 0.4%（W/W） and solid-liquid ratio was of 1：10（W/V）.This study could provide theory evidence and technical support on comprehensive utilization of pork bones in industrialization.

关 键 词：猪骨 酶解 PROTAMEX复合蛋白酶 工艺 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]