Hartley方法优化菠萝皮渣多酚化合物提取工艺  

Optimizing the Extraction of Polyphenols from Pineapple Peel Residue by Hartley's Experimental Design Method

作　　者：李俶[1,2] 沈佩仪[2] 吴华星[2] 刘成梅[2] 李积华[1] 

机构地区：[1]农业部热带作物产品加工重点开放实验室,广东湛江524001 [2]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2011年第4期131-134,共4页Food Science

摘　　要：在单因素试验基础上,采用SAS9.2中的响应曲面分析-Hartley方法对菠萝皮渣中的多酚类化合物提取工艺进行优化,建立提取温度、提取时间、乙醇体积分数和液料比4个因素与总多酚得率(Y)之间的编码水平回归模型,并得出各因素对菠萝皮渣总多酚得率的影响顺序为提取温度>提取时间>液料比>乙醇体积分数。在提取温度48.4℃、提取时间73.6min、乙醇体积分数41.6%、液料比46.8:1(mL/g)的条件下,菠萝皮渣总多酚得率最高,可达7.77mg/g,与理论值7.80mg/g基本吻合。响应面法所得的优化提取条件工艺参数可靠,可用于菠萝皮渣中多酚产品的开发。In the present study,process optimization for the extraction of polyphenols from pineapple peel residue with ethanol aqueous solution was carried out by Hartley＇s experimental design method combined with response surface analysis based on a series of one-factor-at-a-time experiments. A regression model for total polypenhols yield as a function of four factors,namely temperature,extraction time,ethanol concentration and liquid-to-material ratio was built. The importance of the four factors affecting total polypenhols yield declined in the following sequence：temperature,extraction time,liquid-to-material ratio and ethanol concentration. Extraction at 48.4 ℃for 73.6 min with a 46.8-fold volume of 41.6% ethanol aqueous solution yielded the most total polyphenols,reaching 7.77 mg/g,which was basically in accordance with the model-predicted value. In conclusion,the optimized extraction process is reliable and can be used for the development of products based on pineapple peel polyphenols.

关 键 词：菠萝皮渣 多酚 响应曲面 Hartley方法 提取 

分 类 号：R284.2[医药卫生—中药学]