低聚半乳糖糖基化对β-乳球蛋白诱导产生IgE的抑制作用  

Conjunction with galactooligosaccharide suppressed IgE production by β-Lactoglobulin

作　　者：徐晋予[1] 刘宁宁[1] 夏薇[1] 杨铮[1] 许美玉[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《食品科技》2011年第2期5-8,共4页Food Science and Technology

基　　金："十一五"国家科技支撑计划子课题(2006BAD04A06);教育部<留学回国人员科研启动基金>项目;北京林业大学国家大学生创新性实验计划院级项目

摘　　要：用低聚半乳糖对牛乳β-乳球蛋白进行糖基化处理,通过间接ELISA以及动物试验检测该结合产物的抗原性和免疫原性,结果表明糖基化处理能有效降低牛乳β-乳球蛋白的抗原性和免疫原性。通过动物试验和双抗体夹心ELISA方法检测牛乳β-乳球蛋白诱导产生IgE的量,结果表明低聚半乳糖糖基化对牛乳β-乳球蛋白诱导产生IgE具有显著抑制作用。Bovine milk β-Lactoglobulin was conjugated with galactooligosaccharide.Antigenicity and immuno-genicity of the conjugates were measured by indirect ELISA assay and animal experiments.The results showed that conjugation with galactooligosaccharide can reduce the antigenicity and immunogenicity of bovine milk β-Lactoglobulin effectively.Besides,animal experiments and sandwich ELISA method were used to measure the IgE level in vivo,induced by β-LG and its glycosylation product.The result indicated that conjunction with galactooligosaccharide suppressed IgE production by bovine milk β-Lactoglobulin significantly.

关 键 词：牛乳β-乳球蛋白 糖基化 低聚半乳糖 IGE 

分 类 号：Q511[生物学—生物化学]