白果过敏蛋白及其过敏原性研究  

Identification and Allergenicity of Allergic Proteins in Ginkgo Seeds

作　　者：李莹莹[1] 吴彩娥[1] 杨剑婷[2] 范龚健[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]安徽科技学院食品药品学院,安徽凤阳233100

出　　处：《食品科学》2011年第7期16-20,共5页Food Science

基　　金：教育部博士点基金项目(200802980004)

摘　　要：对白果的主要过敏蛋白进行鉴定,并对其过敏原性进行分析。以SDS-PAGE和Western-Blotting分析鉴定白果蛋白提取液中的蛋白质组分和过敏蛋白,通过间接ELISA法检测过敏小鼠血清中sIgE的效价。白果的蛋白质条带主要有13条,其中以21kD和32kD含量最多。Western-Blotting分析结果表明,白果蛋白中存在3条过敏原蛋白,分子质量分别为21、32、36kD。当酶标二抗稀释度为1:1000,白果蛋白包被质量浓度为50μg/mL,间接ELISA测得过敏小鼠血清中sIgE的效价为1:6400。对白果蛋白进行分析鉴定,明确了白果中存在的过敏原蛋白。SDS-PAGE and Western-Blotting were used to identify the protein composition and allergic proteins of ginkgo seed extract,respectively.A total of 13 bands were observed in the SDS-PAGE pattern,and the fractions of 21 kD and 32 kD exhibited the highest content.Western-Blotting analysis indicated that ginkgo seeds had 3 allergic proteins,whose molecular weights were 21,32 kD and 36 kD,respectively.When the enzyme secondary antibody concentration was 1：1000 and the ginkgo protein coating concentration was 50μg/mL,the serum IgE titer of mice allergic to ginkgo seeds was detected to be 1：6400 by indirect ELISA.

关 键 词：白果 过敏蛋白 SDS-PAGE Western-Blotting 间接ELISA 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]