纳滤技术浓缩纯化玉米肽及对其醒酒活性的影响  

Nanofiltration for Concentration and Purification of Corn Peptides and Their Effects on Antialcoholism Activity

作　　者：于国才[1] 何慧[1] 靳桢[1] 黄文浩[1] 李江涛[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2011年第8期10-14,共5页Food Science

基　　金：国家自然科学基金项目(30972043);国家"863"计划项目(2008AA10Z314)

摘　　要：探讨纳滤技术浓缩纯化玉米肽的过程中肽的醒酒活性的变化。本研究选用截留分子质量160D和360D的两种纳滤膜,对pH8.0玉米肽液(Mw<5kD)进行浓缩纯化,比较两种膜对其中主要成分的传质效果,并进一步探讨透析过滤过程对玉米肽醒酒活性的影响。结果表明,玉米肽液浓缩过程中,相对于360D纳滤膜,160D纳滤膜对肽类、氨基酸和Na+均具有较低的传质效率,但其对玉米肽的截留率却在88.80%以上,脱盐效果与360D膜相当,经3次透析后,脱盐率达94.6%,且160D纳滤膜在透析过程中很好地保持玉米肽对乙醇脱氢酶(ADH)的激活作用。结论:宜选用160D纳滤膜对玉米肽液进行浓缩纯化,透析过滤3次。Nanofiltration membranes（NFM）with molecular weight cut off（MWCO）160 D and 360 D were used to concentrate and purify corn protein hydrolysate of MW5 kD at pH 8.0.The mass transfer efficiencies of peptides,amino acids and sodium ion（Na＋）in concentration process and the changes in hydroxyl free radical scavenging activity and antialcoholism activity of corn peptides（CPs）in dialysis process were evaluated.The results showed that although 160 NFM exhibited lower transmission/ rejection rates of CPs,amino acids and Na＋as compared to 360 D NFM,the rejection rate of CPs by 160 D NFM was above 88. 80%and it provided desalting effect similar to that of 360 D NFM and yielded a desalting efficiency of 94.6%after triple repeated dialysis.Moreover,the activating effect of corn peptides on alcohol dehydrogenase（ADH）was kept very well in 160 D NFM dialysis.In general,160 D NFM is suitable to be used for the concentration and purification of corn protein hydrolysate and the number of repeated dialysis should be no more than 3.

关 键 词：纳滤 玉米肽 醒酒 透析 脱盐 乙醇脱氢酶 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]