脉冲电场提取西藏灵菇胞外多糖条件优化  

Optimization of exopolysaccharide extraction process from Tibetan spiritual mushroom by pulsed electric fields

作　　者：张铁华[1] 王少君[1] 刘迪茹[1] 袁媛[1] 于亚莉[1] 殷涌光[2] 

机构地区：[1]吉林大学军需科技学院,长春130062 [2]吉林大学生物与农业工程学院,长春130022

出　　处：《吉林大学学报（工学版）》2011年第3期882-886,共5页Journal of Jilin University:Engineering and Technology Edition

基　　金：吉林省科学技术厅项目(20090225);吉林大学博士启动基金项目(20080017)

摘　　要：以西藏灵菇发酵液为材料,通过高压脉冲电场技术研究了辅助提取胞外多糖的最优条件。结果表明:高压脉冲电场辅助提取乳酸菌胞外粗多糖的最佳技术条件是:电场强度为40kV/cm,脉冲数为8,pH值为7。影响胞外多糖提取的因素的主次顺序为电场强度>pH>脉冲数,其中电场强度对胞外多糖提取具有显著性影响(α<0.05)。优化处理后胞外多糖提取数量比对照组提高了84.30%。The high intensity pulsed electric field-assisted extraction technique was used to optimize the conditions of exopolysaccharides （EPS） extraction in Tibetan spiritual mushroom broth. The results show that the optimal conditions of this extraction technique are： electric field intensity of 40 kV/cm, number of pulses of 8, and pH of 7. These factors affect the EPS extraction in the order of electric field intensity〉 pH 〉 number of pulses. The influence of the electric field intensity on EPS extraction is significant （α〈 0.05）. Under the optimal conditions the EPS extraction was increased by 84.3% compared to that of the control group.

关 键 词：食品加工技术 脉冲电场 西藏灵菇 胞外多糖 提取 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]