基于脲酶催化和酸碱指示剂变色原理的高脲乳快速检测方法的建立  

Rapid Detection Method for High-Urea Milk Based on Urease Catalysis

作　　者：杨水云[1] 郑潇[1] 韩禹[1] 隆永秋[1] 刘永青[1] 

机构地区：[1]西安交通大学生命科学与技术学院线粒体医学研究所生物医学信息工程教育部重点实验室,陕西西安710049

出　　处：《食品科学》2011年第10期222-226,共5页Food Science

摘　　要：针对市场上存在的高脲乳(掺假和病理性)问题,建立区别于正常生理浓度范围的快速检测方法。检测方法依据尿素被酶解导致的pH值升高幅度与其浓度呈正相关这一机理,对牛乳起始pH值进行优化调整为6.8时,选择溴百里酚蓝作为指示剂,可以使乳脲含量达40mg/100mL生理上限卡切值以上时变色,从而区别检出高脲问题乳。研究表明,依此组装的高脲乳检测试剂盒,可用于生产实践。The objective of this study is to establish a rapid detection method for milk with urea content exceeding physiological range.Based on the fact that pH increase could result from urea enzymolysis positively correlated with urea concentration.Bromothymol blue was chosen as the acid-base indicator to determine high-urea milk.The indicator can develop color from yellow-brown to green-blue when urea content was higher than 40 mg/100 mL.On the basis of our present study,the developed reagent kit for the determination of high-urea milk is useful for practical application.

关 键 词：尿素掺假乳 病理性高脲乳 检测试纸 检测试剂盒 脲酶 乳脲氮 

分 类 号：TS201.6[轻工技术与工程—食品科学]