酿酒酵母SC408转化3-甲硫基丙醇条件的研究  

Study on Conditions of Bio-transformation of 3-(methylthio)-1-propanol by Saccharomyces Cerevisiae SC408

作　　者：侯晟[1] 赵磊[1] 杨雪莲[1] 王成涛[1] 

机构地区：[1]北京工商大学食品学院/食品添加剂与配料北京高校工程研究中心/食品风味化学北京市重点实验室,北京100048

出　　处：《北京工商大学学报（自然科学版）》2011年第2期18-22,共5页Journal of Beijing Technology and Business University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31071593);国家重点基础研究发展计划973项目(2007CB707802)

摘　　要：研究了一株产3-甲硫基丙醇的产香酵母菌Saccharomyces cerevisiae SC408的培养基组分和转化条件.均匀试验设计优化后的培养基组分为:氨基酸4.0 g/L、KH2PO48.0 g/L、K2HPO46.0g/L、MgCl20.01 g/L、FeCl20.02 g/L、ZnSO40.03 g/L、酵母膏0.8 g/L、葡萄糖30.0 g/L和NaCl 2.0g/L;接种量和培养条件对3-甲硫基丙醇产生一定影响,在摇床培养30℃,转速200 r/min,SC408发酵132 h,其3-甲硫基丙醇产量达到1.6 g/L,氨基酸的摩尔转化率约为72%.The conditions of bio-transformation of methionol by Saccharomyces cerevisiae SC408 were studied.The optimal culture conditions were obtained as follows： amino acid of 4.0g/L,KH2PO4 of 8.0g/L,K2HPO4 of 6.0g/L,MgCl2 of 0.01g/L,FeCl2 of 0.02g/L,ZnSO4 of 0.03g/L,yeast extract of 0.8g/L,glucose of 30.0g/L,NaCl of 2.0g/L,temperature of 30℃ and shaking rate of 200r/min.The maximum yield of aimed product under the optimal conditions reached 1.6g/L at 132h and the transformation rate was 72%.

关 键 词：SACCHAROMYCES CEREVISIAE 转化 3-甲硫基丙醇 

分 类 号：TS202.3[轻工技术与工程—食品科学]