产谷胱甘肽面包酵母的选育及发酵条件优化研究  

Breading of High Glutathione Producing Saccharomyces erevisiae Strain and Optimization of its Fermentation Conditions

作　　者：吕鸿雁[1] 陈叶福[1] 周大伟[1] 佐一含[1] 肖冬光[1] 

机构地区：[1]天津科技大学生物工程学院工业微生物重点实验室,天津300457

出　　处：《现代食品科技》2011年第5期559-563,共5页Modern Food Science and Technology

摘　　要：本文以面包酵母BV54为出发菌株,镉盐抗性为筛选标记,经紫外和硫酸二乙酯复合诱变,筛选出一株高镉盐抗性面包酵母菌株BV54-11-11,经摇瓶发酵其谷胱甘肽产量达98.8 mg/L,胞内含量为15.22 mg/g。借助于SAS软件,用最陡爬坡路径逼近最大响应区域,再利用Box-Behnken实验设计及响应面分析法进行回归分析,对该菌株摇瓶发酵生产GSH工艺条件进行了优化,优化的发酵条件为:葡萄糖30.15 g/L、装液量49.72 mL/250 mL、摇床转速178.76 r/min,优化后进行发酵实验,菌体产GSH量可达122.02 mg/L,胞内含量为21.44 mg/g,较BV54分别提高107.5%和115.7%。Bread yeast（Saccharomyces cerevisiae） BV54 was screened out by UV and DES mutagenesis,and a mutant strain named as BV54-11-11 with high-yield of glutathione was obtained through cadmium sulfate tolerance plate screening.The glutathione yield of BV54-11-11 in shake-flask fermentation was 98.8 mg/L and GSH content was 15.22 mg/g.With SAS software,the path of steepest ascent tests was used to approach the largest response region,and Box-Behnken experimental design and response surface method were applied to optimize the conditions of GSH fermentation.The optimized fermentation conditions were obtained as follows： glucose 30.15 g/L,liquid volume 49.72 mL/250 mL,and rotation speed 178.76 r/min.Under this optimized conditions,the intracellular GSH production and GSH content of mutant BV54-11-11 were 122.02 mg/L and 21.44 mg/g,respectively,which were 107.5% and 115.7% higher than those of BV54.

关 键 词：谷胱甘肽 面包酵母 诱变 响应面 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]