白骨壤果实7种溶剂提取液的自由基清除作用  

Free radical scavenging activity of seven different extracts from the fruits of Avicennia Marina

作　　者：杨维[1] 夏杏洲[1,2] 韩维栋[3] 谌素华[1] 王维民[1] 

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]华南理工大学轻工与食品学院,广东广州510640 [3]广东海洋大学农学院,广东湛江524088

出　　处：《食品工业科技》2011年第6期102-104,106,共4页Science and Technology of Food Industry

基　　金：广东海洋大学自然科学基金-红树林团队项目(0712117)

摘　　要：用水、95%乙醇、甲醇、丙酮、正丁醇、乙酸乙酯、石油醚为溶剂浸提白骨壤果实,分别采用DPPH法、Fenton反应、邻苯三酚自氧化法测定了7种提取液清除DPPH自由基、羟自由基的能力、超氧阴离子自由基的能力。结果表明:水、甲醇、95%乙醇提取液得率及总黄酮含量最高;7种白骨壤果实提取液对DPPH·、·OH及O-2·三种自由基均显示出一定的清除能力,甲醇、水、95%乙醇三者的提取液清除三种自由基的能力及总抗氧化活性较强,正丁醇、丙酮、乙酸乙酯提取液的清除活性和总抗氧化活性次之,石油醚提取液的最弱。The dry fruit of Avicennia Marina was extracted with seven different solvent dipping at normal temperature to get methanol extract,water extract,95% ethanol extract,acetone extract,n-butanol extract ethyl acetate extract and petroleum extract,then their scavenging activities of 2,2-Diphenyl-1-picrylhydrazyl（DPPH）free radical,hydroxyl free radical（·OH）,superoxide free radical（O-2·）were measured by the methods of DPPH,Fenton,pyrograllol auto-oxidation respectively.The results showed higher extraction rate and total flavonoids yield in water extract,methanol extract and 95% ethanol extract,and all seven extracts exhibited certain radical scavenging activities on DPPH·,·OH and O-2·.The antioxidant and radical scavenging activities of water extract,95% ethanol extract and methanol extract was higher than acetone extract,n-butanol extract and ethyl acetate extract,while weak activity in petroleum extract.

关 键 词：白骨壤果实 超氧阴离子 羟自由基 DPPH自由基 

分 类 号：TS201.1[轻工技术与工程—食品科学]