超滤法浓缩菊糖果糖转移酶的初步研究  

Inulin fructotransferase concentration by ultra-filtration method

作　　者：杭华[1] 赵萌[1] 周榴明[1] 沐万孟[1] 缪铭[1] 江波[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品工业科技》2011年第6期201-203,共3页Science and Technology of Food Industry

摘　　要：超滤浓缩的菊糖果糖转移酶粗酶根据用途可直接使用或进一步纯化,采用截留分子量为10kDa的Pellicon-2盒式超滤膜包,对菊糖果糖转移酶超滤浓缩进行了初步研究。结果表明,酶解菊糖产物为DFAIII;酶收率85%以上,膜通量减少至3.2L/m2·h;单位体积酶活(U/mL)增加8.5倍,比酶活达到6.7U/mg;再生后,膜通量可以恢复95%以上。Inulin fructotransferase concentrated through ultra-filtration could be utilized directly and further purified.Pellicon-2 ultra-filtration membrane bag with molecular weight cut off（MWCO）of 10kDa was investigated in order to concentrate inulase solutions.Results indicated that DFAIII was the enzymatic hydrolysis product.And after concentration,85% enzyme yield was obtained,while membrane flux decreased to 3.2L/m2·h.Furthermore,concentration resulted in an unit volume enzyme activity（U/mL）fold of 8.5 and increased inulase specific activity to 6.7U/mg.Through regeneration,membrane flux could be recovered to over 95% of original level.

关 键 词：菊糖果糖转移酶 超滤 浓缩 

分 类 号：TS201.25[轻工技术与工程—食品科学]