直接竞争酶联免疫分析方法检测猪肉中的沙丁胺醇  

Determination of salbutamol residue in pig muscle by direct competitive enzyme-linked immunosorbent assay

作　　者：郭柏雪[1] 生威[1] 余桂春[1] 石曼[1] 邓捷[1] 陈文洁[1] 王硕[1] 

机构地区：[1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品工业科技》2011年第6期388-390,共3页Science and Technology of Food Industry

基　　金：“十一五”科技支撑课题计划资助项目(2009BADB9B06)

摘　　要：建立了一种用于检测沙丁胺醇的直接竞争酶联免疫分析(ELISA)方法,方法灵敏度(IC50)为0.80μg/L,检测限(IC15)为0.06μg/L,检测的线性范围0.06～20.00μg/L。与克伦特罗的交叉反应率为100%,与特布他林的交叉反应率为10.5%,与其他结构类似物并无明显的交叉反应,因此可以采用本实验建立的直接竞争酶联免疫方法同时测定食品中的沙丁胺醇和克伦特罗。本实验的样品处理方法简便,猪肉样品中的检测限为0.6μg/kg,添加三个浓度的样品,其添加回收率均在85%～100%之间,RSD值小于5.51%。A direct competitive enzyme-linked immunosorbent assays（cd-ELISA）was developed for detection of salbutamol in pork using polyclonal antibody.The sensitivity（IC50）and limit of detection（IC15）of assay were 0.80μg/L and 0.06μg/L,respectively.The ELISA detection system had the linear detection of 0.06～20.00μg/L.All of the test compounds,except clenbutarol（100%）and terbutaline（10.5%）,showed low cross-reactivity.Then this direct competitive ELISA could potentially be applied to the determination of salbutamol and clenbutarol.The limit of detection of this developed ELISA for salbutamol in pork was 0.6μg/kg.The average recoveries were in ranges of 85%～100% and RSD were below 5.51%.

关 键 词：沙丁胺醇 抗体 直接竞争ELISA 猪肉 

分 类 号：TS207.3[轻工技术与工程—食品科学]