超声辅助脂肪酶催化L-抗坏血酸脂肪酸酯的合成  

Synthesis of Ascorbyl Fatty Acid Esters from Lard by Ultrasound-assisted Lipase Catalysis

作　　者：彭杨[1] 穆青[1] 魏微[1] 熊园[1] 刘建伟[1] 陆兆新[1] 吴涛[1] 赵海珍[1] 

机构地区：[1]南京农业大学食品科技学院酶工程研究室,江苏南京210095

出　　处：《食品科学》2011年第12期101-105,共5页Food Science

基　　金：南京农业大学大学生科研训练项目(0908A05)

摘　　要：采用猪油和抗坏血酸为原料,通过单因素试验和正交试验,研究超声作用对酶促转酯化反应合成抗坏血酸脂肪酸酯的影响,并确定其最佳反应条件。结果表明,最佳反应条件为:叔戊醇为溶剂,Novozym 435用量20%(抗坏血酸质量百分比),抗坏血酸与猪油物质的量的比1:3,超声功率350W,超声频率59kHz,反应时间9h,在此条件下,抗坏血酸脂肪酸酯转化率达到88.00%。超声处理不仅可以提高产物产率(从71%提高到88%),还可以大大缩短反应时间(从32h降低到9h)。超声条件下Novozym 435有良好的操作稳定性,反应6批次后,酶活还保持在比较高的水平。One-factor-at-a-time and orthogonal array design methods were employed to optimize transesterification conditions for lipase catalyzed synthesis of scorbyl fatty acid esters from lard and ascorbic acid with ultrasound assistance.The optimum synthesis conditions of scorbyl fatty acid esters were tert-amyl alcohol as reaction medium,Novozym 435 lipase amount of 20%（calculated on the basis of the mass of ascorbic acid）,molar ratio of ascorbic acid to lard of 1：3,ultrasound power of 350 W,ultrasound frequency of 59 kHz and reaction time of 9 h,and the resulting conversion rate of ascorbyl fatty acid esters was up to 88.00%.The ultrasonic treatment could improve scorbyl fatty acid ester yield from 71% to 88% and reduce the reaction time from 32 to 9 h.Novozym 435 exhibited excellent stability under the ultrasonic treatment condition and its activity remained at a relatively high level even after its sixth repeated use.

关 键 词：猪油 脂肪酶 抗坏血酸脂肪酸酯 超声波 转酯化 

分 类 号：Q564[生物学—生物化学]