一株产纤维素酶和植酸酶的米曲霉的诱变筛选和固体发酵  

Solid-State Fermentation and Screening of a Strain of Aspergillus Oryzae Producing Cellulase and Phytase

作　　者：管海华[1] 贾秉晟[1] 王家芳[1] 何瑶[1] 杨启银[1,2,3] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]南京市微生物工程技术研究中心,江苏南京210046 [3]江苏省微生物资源产业化工程技术研究中心,江苏南京210046

出　　处：《南京师大学报（自然科学版）》2011年第2期83-89,共7页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省科技厅项目(BG2005326)

摘　　要：通过紫外诱变和微波辐射米曲霉菌株,筛选出高产纤维素酶和植酸酶米曲霉菌株ZQH48,并进行了固体发酵条件的优化.优化后的培养基配方为:菌渣:麸皮=1∶1,氯化铵12%,蛋白胨2%,硫酸镁0.07%,氯化钠0.5%,磷酸二氢钾0.02%,固水比为1∶1;最佳培养温度35℃.优化后纤维素酶活力可达161.55 U/g,较原始菌株提高5.42倍;植酸酶活力达1 541.13 U/g,较原始菌株提高1.56倍.The strain of Aspergillus Oryzae ZQH48 overproducing cellulase and phytase was isolated by UV and microwave radiation,at the same time,the cultural factors and conditions of solid-state fermentation were also optimized.The optimal culture condition was 12%ammonium chloride,2%peptone,0.07%magnesium sulfate,0.5%sodium chloride,0.02%potassium dihydrogen phosphate,ratio of liquid to material and mushroom dregs to bran 1∶1,35℃.In this condition,the activity of cellulase and phytase of the ZQH48 strain increased by 161.55 U/g and 1 541.13 U/g respectively,which was 5.42 times and 1.56 times higher than the parent strain.

关 键 词：菌渣 米曲霉 纤维素酶 植酸酶 固体发酵 

分 类 号：Q936[生物学—微生物学]