转高赖氨酸融合蛋白基因大米喂养SD大鼠90天试验研究  

A 90-Day Toxicology Study of Transgenic Rice Expressing Lysine-Rich Protein Fusion Gene in Sprague-Dawley Rats

作　　者：董英[1] 施卫东[1] 周兴华[1] 张奕[1] 王云[1] 肖香[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《中国农业科学》2011年第13期2768-2776,共9页Scientia Agricultura Sinica

基　　金：国家转基因生物新品种培育重大专项(2009ZX-08011-003B)

摘　　要：【目的】利用大鼠食用转高赖氨酸融合蛋白基因大米(简称转GL基因大米)试验来初步评价转GL基因大米的食用安全性。【方法】根据转GL基因大米及其亲本大米的营养成分,分别以70%的大米添加量配制成全价料,配制饲料营养成分与对照组饲料一致。将刚断乳的SD大鼠按性别和体重分为3组:转GL基因大米组、亲本大米组和对照组。分别饲喂相应组饲料90 d,自由进食。观察大鼠体重、进食量、食物利用率、血常规、血生化、尿常规、脏器系数和脏器的病理学检查。【结果】转GL基因大米组所有检测指标与亲本大米组相比均无统计学差异(P>0.05);转GL基因大米组与对照组相比,转GL基因大米雌性组进食量、雄性组试验末期血清中CHOL显著低于对照组(P<0.05),而转GL基因大米雌性组食物利用率、雄性组试验末期全血中MCV、雄性组试验中期血清中AST、雄性组试验末期血清中AST/ALT和P显著高于对照组(P<0.05),但这些数据并没有显示出生物学意义上的显著性差异,而且血常规与血生化指标均在正常值范围内。【结论】转GL基因大米对大鼠生长发育无明显不良影响,转GL基因大米和其亲本大米对大鼠有同等的食用安全性。[ Objective ] Rats were fed by transgenic rice expressing lysine-rich protein fusion gene to study if the transgenic rice possesses potential toxic or adverse effects. [Method] According to the composition of the transgenic GL gene rice and its parental rice, a nutritionally balanced purified diet with 70% transgenic GL gene rice or 70% parental rice was prepared. And the nutrition of the diet was the same as the control diet. Sixty weanling Sprague-Dawley rats were divided into three groups according to their sex and weight respectively： transgenic rice group, nontransgenic rice group, and control group. They were fed with corresponding diet for 90 days. Indicators including body weight, feed volume, feed utilization rate, blood routine test, blood biochemistry test, urinary routine test, relative organ weights and organ histopathological examinations were observed. [Result] No significant differences were found between transgenic rice group and non-transgenic rice group （P〉0.05） and no histopathological damage was detected. Female feed volume and CHOL of male serum of terminal experiment in transgenic rice group were lower than those of control group （P〈0.05）. Female feed utilization rate, MCV of male whole blood of terminal experiment, AST of male serum of midterm experiment, AST/ALT and P of male serum of terminal experiment were higher than those of control group （P〈 0.05）, but biological differences were not observed, and the values of all the blood routine parameters and serum parameters were still within the normal ranges of values for rats of this age and sex. [Conclusion] Transgenic rice has no adverse effects on rats, and the transgenic rice is as safe and wholesome as its parental rice.

关 键 词：转基因大米 赖氨酸 稻米 安全评价 

分 类 号：S865.12[农业科学—野生动物驯养] S511[农业科学—畜牧兽医]