由马铃薯淀粉制得磷酸寡糖的定性定量测定  

Determination of Phosphorylated Oligosaccharides Prepared from Potato Starch

作　　者：杨丽[1] 杨文军[1] 刘霞[1] 杜先锋[1] 

机构地区：[1]安徽农业大学茶与食品科技学院,安徽合肥230036

出　　处：《食品科学》2011年第14期198-201,共4页Food Science

基　　金：国家"863"计划项目(2006AA10Z340)

摘　　要：对以马铃薯淀粉为原料、全酶法制得的磷酸寡糖进行定性定量分析。通过采用薄层层析法(thin layerchromatography,TLC)和高效阴离子交换色谱-脉冲安培检测(high-performance anionic exchange chromatography-air puls-ing ampere detectors,HPAEC-PAD)对磷酸寡糖组分进行检测分析,同时利用红外吸收光谱(infrared spectroscopy,IR)对其进行结构分析。结果表明:磷酸寡糖是结合有磷酸基团的并且聚合度在3～7之间的麦芽低聚糖混合物,以麦芽三糖至麦芽六糖组分居多。The current study was carried out to quantitatively and qualitatively analyze phosphorylated sligosaccharides prepared from potato starch by totally enzymatic method. The phosphorylated sligosaccharide composition was determined by thin layer chromatography （TLC） and high performance anionic exchange chromatography with pulsed amperometric detection （HPAEC-PAD）. Infrared spectroscopy （IR） was used for structure elucidation. The analytes were identified as a mixture of maltooligosaccharides containing phosphate groups and having a degree of polymerization between 3 and 7, in which maltotriose, maltotetraose, maltopentaose and maltohexaose were the dominant components.

关 键 词：磷酸寡糖 薄层层析法 高效阴离子交换色谱-脉冲安培检测 红外吸收光谱 

分 类 号：TQ917[化学工程]