一株法夫酵母虾青素高产菌株的生产性能  

Characterization and evaluation of an astaxanthin over-producing Phaffia rhodozyma

作　　者：倪辉[1,2,3] 洪清林[1] 肖安风[1,2] 李利君[1] 蔡慧农[1,2,3] 苏文金[1,2,3] 

机构地区：[1]集美大学生物工程学院,厦门361021 [2]福建省高校食品微生物与酶工程技术研究中心,厦门361021 [3]厦门市食品与生物工程技术研究中心,厦门361021

出　　处：《生物工程学报》2011年第7期1065-1075,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.20702019);福建省产学研重大专项项目(No.2010N5009);集美大学科研创新团队基金(No.2010A006)资助~~

摘　　要：为了评价虾青素高产菌株-法夫酵母JMU-MVP14的生产性能及建立虾青素高产发酵技术,通过测定糖、生物量、虾青素产量、总类胡萝卜素产量等发酵参数,用摇瓶试验对比了法夫酵母JMU-MVP14和出发菌株的差异,用7 L罐试验对比了pH值调控方式及补料培养基成分对发酵的影响,用1 m3罐试验评估了法夫酵母JMU-MVP14高密度发酵虾青素的产量水平。摇瓶发酵结果表明,法夫酵母JMU-MVP14虾青素及总类胡萝卜素的细胞产率分别达到6.01 mg/g及10.38 mg/g;7 L罐分批发酵试验结果表明,自动流加调控pH值比间歇调控pH更有利于该法夫酵母的生长及虾青素合成;法夫酵母JMU-MVP14的μmax和Ks分别为0.20 h?1及21.73 g/L,其虾青素合成的模型为部分生长关联型。7 L罐补料分批发酵试验表明,在发酵过程中只补充糖液而不补充酵母膏及玉米浆有利于高密度、高产发酵虾青素;7 L罐补料分批发酵的生物量、虾青素和总类胡萝卜素的体积产率、虾青素和总类胡萝卜素的细胞产率分别达到了32.81 g/L、155.99 mg/L、399.99 mg/L、4.94 mg/g、12.19 mg/g。1 m3罐中试补料分批发酵的生物量、虾青素和总类胡萝卜素的体积产率、虾青素和总类胡萝卜素的细胞产率分别达到85.11 g/L、279.96 mg/L、618.01 mg/L、3.29 mg/g和7.26 mg/g;法夫酵母JMV-MVP14细胞中的蛋白质、总糖、脂肪含量分别为21.54%、41.34%和34.31%。该菌株具有很强的虾青素生产能力及良好的综合利用价值,能大幅度提高虾青素的发酵生产水平。We evaluated an astaxanthin overproducing Phaffia rhodozyma JMU-MVP14,and developed astaxanthin high-yielding fermentation process.We analyzed several fermentation parameters,i.e.,biomass,astaxanthin and total carotenoids content to compare the characteristics of P.rhodozyma JMU-MVP14 and the original strain through flask fermentation experiments.We conducted batch and fed-batch fermentation experiments in 7 L fermentor to investigate the effects of pH controlling models and feeding medium compositions on the production of astaxanthin.We further evaluated the capability and practical value of P.rhodozyma JMU-MVP14 by fed-batch cultivation in the 1 m3 fermentor.Flask fermentation experiments revealed that P.rhodozyma JMU-MVP14 produced high yield of astaxanthin and carotenoids with specific productivity of astaxanthin and specific productivity of total carotenoids of 6.01 mg/g and 10.38 mg/g.Results of batch culture experiments in the 7 L fermentor showed that controlling the pH by ammonia auto-feeding was better than discontinuously adjusting pH value at 6.0 with regard to the high productivities of biomasses and astaxanthin.This P.rhodozyma strain synthesized astaxanthin partially linked to the growth with the Ks and μmax of 0.20 h？1 and 21.73 g/L,respectively.Results of batch-fed fermentations in 7 L fermentor indicated that the complex feeding medium consisted of 50% glucose,0.5% yeast extract and 0.3% corn steep syrup had lower astaxanthin productivity than the simple feeding medium containing only 50% glucose,which produced biomass,volumetric productivity of astaxanthin,volumetric productivity of total carotenoids,specific productivity of astaxanthin and total carotenoids at 32.81 g/L,155.99 mg/L,4.94 mg/g,399.99 mg/L and 12.19 mg/g,respectively.As fed-batch cultured in 1 m3 fermentor,P.rhodozyma JMU-MVP14 yielded 85.11 g/L of biomass,279.96 mg/L of volumetric productivity of astaxanthin,618.01 mg/L of volumetric productivity of total carotenoids,3.29 mg/g of specific productivity of astaxanthin and 7.26

关 键 词：法夫酵母 虾青素 高产菌株 动力学 补料分批发酵 

分 类 号：TQ929[轻工技术与工程—发酵工程]