基于SYBR Green Ⅰ熔解曲线分析的食品中转基因检测方法  

A SYBR Green Ⅰ-based Real-time PCR Combined with Melting Curve Analysis for GMO Detection in Food

作　　者：张清平[1] 刘洋[1] 曲勤凤[1] 熊薇[1] 葛宇[1] 曹程明[1] 

机构地区：[1]上海市质量监督检验技术研究院,上海200233

出　　处：《食品工业》2011年第7期86-89,共4页The Food Industry

摘　　要：研究建立了一种基于SYBR Green Ⅰ的实时荧光PCR方法,结合熔解曲线分析及位点特异的Tm值辅助判断,可以对不同加工类型及加工程度的食品进行转基因检测。该方法对NOS终止子,RRS,Bt176三个基因位点的检出限达到0.1%,对CaMV35S启动子基因位点的检出限可以达到0.05%。运用该方法对80例不同类型的样品进行检测,检出8例含有转基因成分,其检测结果与传统定性PCR方法和Taqman探针实时荧光PCR方法的结果相符。该方法是一种操作安全方便、成本较低、特异、灵敏的实时荧光PCR方法,适用于食品中转基因成分的定性检测。A SYBR Green Ⅰ-based real-time PCR method combined with melting curve analysis and site-specific Tm analysis was established to detect GMO ingridient in different types of food.The detection limit of this method is 0.1% for the NOS,RRS and Bt176,or 0.05% for the CaMV35S promoter.Eight out of 80 samples tested showed signs of GMO ingredient,which is consistent with the results of the conventional qualifying PCR and the Taqman real-time PCR.This method is advantageous in labor,cost,sensitivity and specificity.It can be applied to the qualitative detection of GMO ingridient in food.

关 键 词：熔解曲线 SYBR Green Ⅰ 转基因 定性检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]