蛹虫草固体培养菌质粗多糖提取与分离工艺研究  

Research on Extraction and Separation of Polysaccharides from Solid-state Fermentation Substance of Cordyceps militaris

作　　者：高宇[1,2] 程俊文[2] 贺亮[2] 吴学谦[2] 李玉[1] 付立忠[2] 胡传久[2] 

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,长春130118 [2]浙江省林业科学研究院浙江省森林资源生物与化学利用重点实验室,杭州310023

出　　处：《中国林副特产》2011年第4期1-4,共4页Forest By-product and Speciality in China

基　　金：浙江省创新团队建设与人才培养项目(2009F20019);浙江省重大攻关项目(2006C12094);浙江省林业科技项目(07A07)的部分研究内容

摘　　要：为探明蛹虫草固体发酵新型培养基菌质中多糖的含量及结构,并探明回流提取方法对此类培养基中多糖提取的条件。采用正交实验确定最佳提取条件,采用苯酚-硫酸法测定菌质中的多糖含量,并用阴离子交换柱进一步分离粗多糖。结果:回流提取的最佳条件为80℃下,料液比1?20回流提取2h,提取次数为3次。在此条件下多糖含量为6.93%。其DPPH清除率可达50%以上,粗多糖经阴离子柱分离得到3种不同的糖蛋白。结论:该实验方法操作简易,条件易控制,结果精准,适合此种培养基中蛹虫草多糖的测定及分离纯化。In order to find out a method for extraction and determination of polysaccharides from Cordyceps militaris and research the conditions of extraction by backflow. The best conditions for extraction of polySaccharides was confirmed by orthogonal experiment, phenol-sulphuric acid was used to determine the contents of polysaccharides, then continue fractionation Polysaccharides by DEAE-Sepharose Fast Flow. Results The result showed the extraction temperature, time, the ratio of raw material to water and times were 80℃, 2h, 1：20 ,3. respectively, the yield reached the maximum 6.93% ,then we get three glucoprotein by DEAE-Sepharose Fast Flow. Conclusion The method is simple,accurate and relia- ble,which is fit for extractration and determination of polysaccharides in Solid-state fermentation of Cordyceps militaris grown on Germinated.

关 键 词：蛹虫草 固体发酵 虫草菌质 多糖 

分 类 号：TS201.25[轻工技术与工程—食品科学]