环氧氯丙烷交联淀粉的制备及其体外消化性能的研究  

Study on preparation and digestibility in vitro of crosslinked starch by epichlorohydrin

作　　者：闵玉涛[1] 宋彦显[1] 马凤青[2] 马庆一[2] 

机构地区：[1]中州大学,河南郑州450044 [2]郑州轻工业学院,河南郑州450002

出　　处：《食品工业科技》2011年第8期261-263,422,共4页Science and Technology of Food Industry

摘　　要：以环氧氯丙烷为交联剂,制备土豆交联淀粉。用正交实验考察环氧氯丙烷用量、氢氧化钠用量、反应温度和反应时间四个因素对交联度的影响,确定最佳工艺条件,并对体外消化条件进行优化,对不同交联度淀粉的消化速度进行研究。体外淀粉消化条件的优化实验显示,消化产物测定的最佳条件为:波长485nm,显色温度100℃,显色时间30min,葡萄糖浓度范围0～80μg/mL,其回归方程为y=0.0044x-0.005,R2=0.9988,且样品溶液在2h内显色稳定。各因素对交联淀粉制备影响的重要性依次为环氧氯丙烷用量、氢氧化钠用量、温度及反应时间;最佳工艺条件为:以50g土豆淀粉计,环氧氯丙烷用量为1.00mL,氢氧化钠用量为0.75g,温度50℃,时间6h,土豆交联淀粉和交联前相比,消化性降低了13.7%～34.5%,且与交联度呈负相关。With epichlorohydrin as crosslinking agent,potato crosslinked starch was prepared.Influences of epichlorohydrin dosage,sodium hydroxide dosage,reaction temperature and reaction time were researched using orthogonal test,and optimum technological condition was determined.The digestibility was also studied.The results showed that the optimum determination condition of crosslinked starch was as follows：wavelength 485nm,temperature 100℃,time 30min,glucose concentration 0 ~ 80μg/mL,regression equation was y=0.0044x-0.005,R2=0.9988,and the sample color was stable within 2h.The factors influencing order was epichlorohydrin dosage,sodium hydroxide dosage,reaction temperature and reaction time.The optimal preparation condition was as follows：50g potato starch as material,epichlorohydrin dosage 1.00mL,sodium hydroxide dosage 0.75g,temperature 50℃,time 6h.under the condition above,the digestibility of crosslinked starch decreased by 13.7% ~34.5% than raw starch,negative correlation with crosslinking degree.

关 键 词：环氧氯丙烷 交联淀粉 体外消化 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]