影响植物乳杆菌A6-1F产亚油酸异构酶条件的研究  

Optimize the production conditions of linoleate isomerase by Lactobacillus plantarum A6-1F

作　　者：赵宏伟[1] 李淑荣[1] 孟宪军[2] 吕加平[1] 张树文[1] 孟倩[1] 

机构地区：[1]中国农业科学院农产品加工研究所,农业部农产品加工质量控制重点开放实验室,北京100193 [2]沈阳农业大学食品学院,沈阳110161

出　　处：《食品科技》2011年第9期45-49,共5页Food Science and Technology

基　　金：国家高技术研究发展计划项目(2006AA10Z345)

摘　　要：诱变选育的高产菌株A6-1F的适宜产酶条件为:种子液中添加0.4mg/mL的LA进行诱导,种子液的菌龄20h,底物浓度0.75mg/mL,接种量4%,pH7.0,培养时间32h。在适宜的条件下,亚油酸异构酶的活性为337.4U/mL。A new strain named A6-1F was screened from original strains maintained in our lab by mutagenesis. The optimal conditions for linoleate isomerase production in MRS medium were as follows： seeds should be induced through adding linoleic acid（concentration was 0.4 mg/mL）, the age of the seeds was 20 h, concentration of substrate was 0.75mg/ mL, inoculum size was 4%, pH was 7.0, incubation time was 32 h. Under this optimal conditions, the activity of linoleate isomerase was 337.4 U/mL.

关 键 词：植物乳杆菌 亚油酸异构酶 酶活 

分 类 号：TS201.3[轻工技术与工程—食品科学]