产核糖核酸酶PA110-7菌株的筛选与初步鉴定  

Isolation and Primary Identification of Ribonuclease-producing Strain PA 110-7 from Suaedae glaucae Bunge

作　　者：矫玉翠[1] 生吉萍[1] 郑嫣燕[1] 王正荣[1] 葛佳[1] 申琳[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2011年第17期246-249,共4页Food Science

基　　金：国家公益性行业(农业)科研专项(200803033)

摘　　要：为筛选产核糖核酸酶并且具有抗病毒活性的植物来源的菌株,并鉴定。本实验从健康的碱蓬中分离到50株益生菌,通过平板显色法筛选得到9株产核糖核酸酶的益生菌,通过噬菌斑法进行复筛得到抗噬菌体率高达69%的菌株110-7。根据菌株的菌落形态、大小、颜色,革兰氏染色、芽孢染色等观察菌体形态以及生理生化实验、16S rDNA序列测定进行鉴定,初步判定该菌株为苏云金芽孢杆菌(Bacillus thuringiensis)。In order to screen and identify ribonuclease-producing strain and evaluate its antiviral activity,50 probiotics from healthy Suaedae glaucae Bunge were isolated and 9 ribonuclease-producing strains were screened by color plate method.The inhibitory activity of strain PA110-7 against Escherichia coli was 69%.Based on the analyses of colony morphology,size and color,Gram staining,spore staining,physiological-biochemical characterization and 16S rDNA sequencing,strain PA110-7 was identified as Bacillus thuringiensis.

关 键 词：碱蓬 益生菌 核糖核酸酶 分离 鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]