食品中鲨鱼源性成分真实性PCR鉴别研究  

Study on authentication of shark derived material in food using PCR

作　　者：郭云霞[1,2] 包建强[1] 张舒亚[2] 陈颖[3] 黄文胜[3] 吕蓉[2] 李想[2] 高琴[2] 刘月明[2] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海出入境检验检疫局,上海200135 [3]中国检验检疫科学研究院,北京100025

出　　处：《食品工业科技》2011年第10期421-424,共4页Science and Technology of Food Industry

基　　金：国家质检总局科研项目(2010IK191);出入境检验检疫行业标准制定项目(2009B172)

摘　　要：建立食品中真实性鲨鱼源性成分PCR鉴别方法,该方法特异性强,灵敏度高。运用普通PCR技术对9种鲨鱼及42种常见非鲨鱼类动植物样品进行检测,9种鲨鱼中出现180bp的特异性扩增条带,其他非鲨鱼样品中均未出现扩增条带,实验表明,本检测方法具有特异性,检测限为0.1ng/μL鲨鱼DNA和0.1%(W/W)鲨鱼肉粉。运用建立的方法对20种常见的鲨鱼产品进行PCR检测,除仿鱼翅和鲨鱼肝油外,所有产品中均能检测出鲨鱼成分。该检测方法能够用于食品中鲨鱼源性成分的真实性鉴别。A specific and sensitive PCR method for identification of shark derived material in food was developed. A 180bp fragment was amplified form 9 kinds of shark samples,whereas no amplification was obtained from other 42 kinds of common plants and animals. The detection limit of the method was 0.1ng/uL DNA concentration and 0.1% （W/W）shark meat powder. 20 commercial shark products samples were analysized by PCR and all the products indicated exist of shark DNA except the false shark fin and shark liver oil. The detection method can be used to the identification of shark derived material in food.

关 键 词：食品 鲨鱼源性成分 鉴别 PCR 

分 类 号：Q789[生物学—分子生物学]