反相高效液相色谱法同时测定马铃薯块茎中酚酸类物质  

Simultaneous Determination of Phenolic Acids in Potato Tuber by RP-HPLC

作　　者：骆成尧[1] 印遇龙[1,2] 阮征[1] 欧阳崇学[1] 文红艳[1] 周笑犁[1] 彭彰智[1] 

机构地区：[1]食品科学与技术国家重点实验室南昌大学生命科学与食品工程学院,江西南昌330031 [2]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《食品科学》2011年第18期300-303,共4页Food Science

基　　金：南昌大学"赣江学者奖励计划"项目

摘　　要：目的:运用反相高效液相色谱法同时测定马铃薯中的绿原酸、咖啡酸、对香豆酸和阿魏酸。方法:色谱柱为YMC-C18(250mm×4.6mm,5μm),流动相为甲醇-水-冰醋酸(30:70:1,V/V)等度洗脱,柱温25℃,检测波长327nm。结果:绿原酸、咖啡酸、对香豆酸和阿魏酸在进样质量浓度分别为7.80～1000、0.78～100、0.312～40、0.078～10μg/mL范围内线性关系良好(r值范围0.9968～0.9999),平均回收率为92.38%～101.73%。结论:本法分析快速准确、灵敏度高、重现性好,江西、北京和新疆3个地域的环境条件对马铃薯的酚酸含量有一定的影响。Objective： A reversed-phase high performance liquid chromatographic（RP-HPLC） method was developed to determine the contents of chlorogenic acid,caffeic acid,p-coumaric acid and ferulic acid in potato tuber from Jiangxi,Beijing and Xinjiang.Methods： The contents of phenolic compounds were measured by RP-HPLC using YMC-C18 column（250 mm × 4.6 mm,5μm） as the sationary phase and CH3OH： H2O： CH3COOH（30：70：1,V/V） as the mobile phase at a flow rate of 1.0 mL/min.Results： A good linear relationship of this method was achieved in the ranges of 7.80-1000μg/mL for chlorogenic acid,0.78-100μg/mL for caffeic acid,0.312-40μg/mL for p-coumaric acid and 0.078-10μg/mL for ferulic acid with a correlation coefficient of 0.9968-0.9999.The average recovery rates of this method were 92.38%-101.73%.Conclusion： The developed RP-HPLC method is characteristic of fast determination,accurate results,high sensitivity and good reproducibility.In addition,different growth environments of potato tuber have an important impact on the contents of phenolic acids.

关 键 词：马铃薯 块茎 酚酸类物质 反相高效液相色谱法 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.7[轻工技术与工程—食品科学与工程]