多重PCR同时检测常见8种食物过敏原  

Detection of Eight Food Allergens with Multiplex PCR

作　　者：王玮[1,2] 韩建勋[1] 袁飞[1] 吴亚君[1] 陈颖[1] 葛毅强[2,3] 

机构地区：[1]中国检验检疫科学研究院,北京1001232 [2]中国农业大学食品学院,北京1000833 [3]中国农村技术开发中心,北京100045

出　　处：《中国食品学报》2011年第6期152-157,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(No.31071552);国家"十一五"科技支撑计划项目(No.2009BADB9B03)

摘　　要：依据大豆Lectin基因,小麦Gliadin基因,花生Arah3基因,腰果Ana o3基因,鱼和虾16S rRNA,牛和鸡线粒体DNA设计特异性引物序列。在单一PCR方法基础上,建立2种4重PCR方法检测8种食物过敏原的技术。该方法检测周期短,具有较好的特异性和灵敏性,可用于对食品中多种食物过敏原的检测和监控。Eight pairs of primers were selected according to the sequence of Lectin gene from soybean,Gliadin gene from wheat,Ara h 3 gene from peanut,Ana o3 gene from cashew,16S rRNA from shrimp and fish,mitochondrial DNA from bovine and chicken respectively,and a multiplex PCR assay for food allergen detection was established on the basis of simple PCR technique.Eight food allergens could be detected rapid,specific and sensitive with 2 multiplex PCR assays at the same time.It could be used for detection and surveillance of many allergens in food.

关 键 词：多重PCR 食物过敏原 检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]