响应面法优化谷胱甘肽发酵培养基的研究  

Optimization of Fermentation Production Medium of Glutathione by Saccharomyces cerevisiae Using Response Surface Methodology

作　　者：周铭锋[1,2] 黄璠[1,2] 张为巍[1,2] 王常高[1,2] 林建国[1,2] 蔡俊[1,2] 

机构地区：[1]发酵工程教育部重点实验室湖北工业大学,武汉430068 [2]湖北工业大学生物工程学院,武汉430068

出　　处：《食品与发酵科技》2011年第5期15-19,30,共6页Food and Fermentation Science & Technology

基　　金：湖北省自然科学基金重点项目;项目编号:2009CDA059

摘　　要：利用Plackett-Burman设计,Box-Behnken中心组合设计和响应面法对酿酒酵母发酵生产谷胱甘肽的培养基进行优化。用Plackett-Burman实验方法研究葡萄糖、酵母粉等5个营养因子对GSH产量的影响,结果表明主要影响因子为葡萄糖、酵母粉、硫酸铵;通过爬坡试验对显著因素进行优化来寻找合成谷胱甘肽产量的最佳值区域,然后用Box-Behnken设计及响应面分析确定主要影响因子的最佳浓度。实验结果表明:在葡萄糖25.95g/L、酵母粉11.47g/L、(NH4)2SO410.16g/L、硫酸镁0.2g/L、磷酸二氢钾2g/L的培养基中,谷胱甘肽产量可达170.459mg/L,比优化前提高了40.65%。To study the optimal fermentation media of glutathione production of Saccharomyces cerevisiae by Plackett-Burman design、Box-Behnken design and Response surface analysis.5 nutrients factors＇effects on the fermention such as glucose,yeast extract etc were evaluated by using Plackett-Burman design.Glucose,yeast extract and（NH4）2SO4 played important roles in GSH production,while others had minor effect.Experimental design of steepest ascent optimize the production medium of glutathione to find the best value area.Box-Behnken design and Response surface analysis response determine the optimal concentration of the main factors.The results of media experiment showed as follow：25.95g/L glucose,11.47g/L yeast extract,10.16g/L（NH4）2SO4,0.2g/L MgSO4,2g/L KH2PO4.Glutathione content was up to 170.459mg/L,which was 40.65% higher than that of before.

关 键 词：谷胱甘肽 酿酒酵母 PLACKETT-BURMAN设计 响应面 

分 类 号：TQ927[轻工技术与工程—发酵工程] TQ936.16