微波结合盐酸羟铵诱变选育高产醋酸菌  

Breeding of high yield acetic acid bacteria by hydroxylamine hydrochloride combined with microwave mutagenesis

作　　者：贺小贤[1] 赵会芳[1] 孙福林[1] 刘苗苗[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《中国酿造》2011年第10期74-77,共4页China Brewing

基　　金：陕西省教育厅自然基金项目(11JK0638)

摘　　要：以醋酸菌AFA-01为出发菌株,首先利用微波诱变菌株,之后再采用盐酸羟铵点式平板法处理,获得产酸性能较好的突变株AFA-WH3,其产酸量为7.35g/100mL,较出发菌株提高了43.55%,乙醇脱氢酶(ADH)活力达2786U/mL,较出发菌株提高94.83%。该菌株经8次传代,产酸稳定,可进行下一步的研究。A high yield of acetic acid mutant strain Acetobacterpasteurianus AFA-WH3 was bred from its parent strain AFA-01 by hydroxylamine hydrochloride combining with microwave mutagenesis. The results showed that the acetic acid production was increased by 43.55%, up to a concentra- tion of 7.35 g/100ml. Moreover, alcohol dehydrogenase（ADH） activities reached 2786U/ml, increased by 94.83%. The strain remained the characteristics of high acetic acid production after 8 times passage, and the mutant strain could be used as experiment strain for further studies.

关 键 词：醋酸菌 微波诱变 盐酸羟铵诱变 乙醇脱氢酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]