飞龙掌血多糖清除自由基活性的研究  

Study on free radical scavenging efficency of Toddalia asiatica polysaccharides

作　　者：田春莲[1] 蒋凤开[2] 文赤夫[2] 

机构地区：[1]林产化工工程湖南省重点实验室,湖南张家界427000 [2]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《食品工业科技》2011年第11期106-108,共3页Science and Technology of Food Industry

基　　金：湖南省科技计划资助项目(2010TP4007-1);湖南省高校"林产资源化学与林化产品开发"科技创新团队支持计划资助(湘教通(2010)212号)

摘　　要：目的:研究飞龙掌血多糖的抗氧化活性。方法:采用Fenton法、DPPH法测定飞龙掌血多糖清除羟基自由基(.OH)、二苯代苦味酰肼自由基(DPPH.)的能力,以VC为对照。结果:质量浓度在0.2～0.4mg/mL范围内,飞龙掌血多糖随浓度增大,清除.OH能力增强,且0.4mg/mL时清除率高达73.7%;质量浓度在5×10-3～5×10-1mg/mL时,对DPPH.的清除率为13.5%～79.5%,清除作用与浓度呈正相关,当浓度为0.5mg/mL时,清除率高达79.5%。结论:飞龙掌血多糖具有较强的体外抗氧化性能,对.OH、DPPH.具有明显的清除作用。Objective：To study the antioxidant activity of polysaccharides from Toddalia asiatica.Methods：The research adopted Fenton method and DPPH method to determine the scavenging ability of Toddalia asiatica polysaccharides to ·OH and DPPH· by comparing with VC.Results：In the concentration of 0.2 ~ 0.4mg/mL,the scavenging ability to ·OH was a positive correlation with the concentration of Toddalia asiatica polysaccharides,when the concentration was 0.4mg/mL,the clearance rate of ·OH was 73.7%.In the concentration of 5×10-3 ~ 5×10-1mg/mL,the clearance rate of DPPH· was 13.5% ~ 79.5%,the scavenging ability to DPPH· was also positively correlated with the concentration of Toddalia asiatica polysaccharides,when the concentration was 0.5mg/mL,the clearance rate of DPPH· was 79.5%.Conclusions：Toddalia asiatica polysaccharides had strong antioxidant properties and possessed a clear scavenging effect to ·OH and DPPH·.

关 键 词：飞龙掌血多糖 羟基自由基 DPPH 抗氧化活性 

分 类 号：TS201.23[轻工技术与工程—食品科学]