高压脉冲电场协同酶法提取牛骨蛋白的研究  

Research on extracting protein from cattle bone by enzymolysis coupled with PEF

作　　者：韦汉昌[1,2] 韦善清[3] 何建华[2] 

机构地区：[1]广西师范大学,广西桂林541004 [2]南宁地区教育学院,广西南宁530001 [3]广西大学,广西南宁530001

出　　处：《食品工业科技》2011年第11期280-282,共3页Science and Technology of Food Industry

基　　金：广西自然科学基金资助项目(桂科自0832105)

摘　　要：以新鲜牛骨为原料,研究高压脉冲电场协同风味酶提取牛骨蛋白。实验结果表明,牛骨粉经高压脉冲电场处理再进行酶解,不仅能够提高骨蛋白溶出率,而且能缩短酶解时间。在电场强度30kV.cm-1、脉冲数25、料液比1∶8、酶用量4000U/g(骨粉质量)、酶解温度50℃、pH6.5等条件下,酶解30min,牛骨蛋白溶出率可达65.2%。The technology of extracting protein from cattle bone by flavor protease coupled with pulsed electric field（PEF）was researched.For extracting protein from cattle bone powder pre-treated by PEF,the results of experiments showed that it not only could make the enzymolysis time short but also increase the dissolution ratio of protein.Under the conditions of electric field intensity 30 kV·cm-1,pulse number 25 times,material to liquid ratio 1∶ 8（g/mL）,enzymes usage 4000U/g,enzymolysis temperature 50℃,pH=6.5 and enzymolysis time 30min,the dissolution ratio of bone protein could reach 65.2%.

关 键 词：高压脉冲电场 酶解 牛骨 蛋白质 提取 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]