Recognition with monoclonal antibodies of a Mr 71000 surface antigen of Plasmodium falciparum merozoites  

作　　者：蒋春雷 徐荻 管惟滨 王笑利 孔宪涛 颜永碧 

机构地区：[1]Department of Parasitology,Second Military Medical University Shanghai 200433 [2]Center of Clinical Immunology,Changzheng Hospital,Second Military Medical University Shanghai 200003 [3]Central Laboratory,Second Military Medical University Shanghai 200433

出　　处：《Journal of Medical Colleges of PLA(China)》1993年第1期65-70,74,共7页中国人民解放军军医大学学报（英文版）

基　　金：The project was supported by the National Natural Science Foundation of China,No 3860869

摘　　要：Merozoite surface antigens can induce protective immune responses and may becandidate antigens for malaria vaccine.Four hybridoma cell lines secreting monoclonalantibodies against Plasmodium falciparum Fcc7801/HN were produced.Antibodies fromthree of the four lines showed significant growth-inhibiting effect on P.falciparum invitro.One monoclonal antibody,known as C6,conjugated the antigen located exclusivelyon the merozoite surface and distributed evenly over the entire surface,as wasdemonstrated by immunoelectron microscopy.C6 also precipitated a single protein of Mr71000.Merozoite surface antigens can induce protective immune responses and may be candidate antigens for malaria vaccine.Four hybridoma cell lines secreting monoclonal antibodies against Plasmodium falciparum Fcc7801/HN were produced.Antibodies from three of the four lines showed significant growth-inhibiting effect on P.falciparum in vitro.One monoclonal antibody,known as C6,conjugated the antigen located exclusively on the merozoite surface and distributed evenly over the entire surface,as was demonstrated by immunoelectron microscopy.C6 also precipitated a single protein of Mr 71000.

关 键 词：Plasmodium FALCIPARUM ANTIBODIES MONOCLONAL MEROZOITE antigens protczoan IMMUNOFLUORESCENCE assay immunoblotting microscopy electron 

分 类 号：R[医药卫生]