植物乳杆菌胞外多糖的脱色及其体外抑瘤效应  

Decolorization and Anti-tumor Activity in vitro of Exopolysaccharides from Lactobacillus plantarum 70810

作　　者：冯美琴[1,2] 张琦[1] 玛依诺.木图拉 黄丽娟[1] 曹林[1] 董明盛[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]金陵科技学院动物科学与技术学院,江苏南京210038

出　　处：《食品科学》2011年第21期107-111,共5页Food Science

基　　金：国家"863"计划项目(2011AA100903)

摘　　要：研究大孔吸附树脂对植物乳杆菌胞外多糖的脱色工艺,并采用Cell Counting Kit-8(CCK-8)法检测植物乳杆菌胞外多糖EPS及单一组分EPS-1、EPS-2对大肠癌细胞株HT-29的体外抑瘤效应。结果表明,选择S-8树脂,树脂用量为4g/100mL,调节胞外多糖溶液的pH值为6,在25℃条件下,糖液质量浓度为2mg/mL,静态吸附4h后脱色率为72.58%,糖保留率为69.15%。植物乳杆菌胞外多糖EPS、EPS-1和EPS-2对大肠癌细胞HT-29的体外增殖均具有显著的抑制作用(P<0.01),且呈现出良好的量效关系。The effects of various operating conditions on decolorization of exopolysaccharides（EPS） from Lactobacillus plantarum 70810 by macroporous resin adsorption were explored in the current work.In addition,the anti-tumor effects of crude EPS from Lactobacillus plantarum 70810 and two fractions thereof obtained by sequential column chromatographies on DEAE-Cellulose and Sephadex G-100,named as EPS-1 and EPS-2,respectively,on large intestine carcinoma cells HT-29 in vitro were tested by cell counting kit-8（CCK-8） assay.The results showed that macroporous resin S-8 provided optimal decolorization of crude EPS from Lactobacillus plantarum 70810 among all resins tested.After 4 h decolorization under the following conditions： macroporous resin S-8 amount 4 g/100 mL,sample pH adjustment until pH 6,25 ℃ and polysaccharide concentration in samples 2 mg/mL,the decolorization rate and retention rate of EPS were 72.58% and 69.15%,respectively.Crude EPS,EPS-1 and EPS-2 exhibited a significant inhibitory effect on the cell growth of large intestine carcinoma cells HT-29 in vitro（P0.01） in a dose-dependent manner.

关 键 词：植物乳杆菌 胞外多糖 大孔吸附树脂 脱色 抑瘤活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]