沙漠生物结皮高产胞外多糖菌株的筛选与鉴定  

Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes

作　　者：王正荣[1,2] 生吉萍[1] 田小磊[1] 武婷婷[1] 刘万振[1] 程凡升[1] 矫玉翠[1] 申琳[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]河南科技学院食品学院,河南新乡453003

出　　处：《食品科学》2011年第21期207-209,共3页Food Science

基　　金：农业部公益性行业(农业)科研专项(200803033)

摘　　要：采用传统的分离纯化方法从沙漠生物结皮中分离到27株产胞外多糖菌株,并通过苯酚-硫酸法从中复筛,筛选到一株产多糖量为7445.80mg/L的菌株XJ-27。根据菌株的形态学特征、生理生化鉴定方法初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis);通过16S rDNA基因序列分析对该菌株进行了鉴定,PCR扩增得到1452bp的序列,PCR产物序列通过Blast软件在NCBI网站中进行同源性比较,通过MEGA4.0软件绘制系统发育树,结果进一步确认了该菌的地位,XJ-27在细菌系统发育分类学上也归属为苏云金芽孢杆菌。Twenty-seven stains producing exopolysaccharides were isolated from biological soil crusts in the Gurbantunggut Desert,Xinjiang by the traditional method.A strain with 7445.80 mg/L exopolysaccharide-producing activity was picked out of them by phenol-sulfuric acid method and named as XJ-27.Based on phenotypic and physiological-chemical characterization,XJ-27 was primarily identified as Bacillus thuringiensis and further confirmed through 16S rDNA sequence analysis,resulting in a 1452 bp PCR amplification sequence,homology comparison with NCBI databases using the software Blast and phylogenetic tree construction using the software MEGA 4.0.

关 键 词：沙漠生物结皮 胞外多糖 鉴定 苏云金芽孢杆菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]