2株酵母菌单倍体原生质体形成与再生条件的研究  

The formation and regeneration of protoplast from two high-alcohol-yield Saccharomyces cerevisiae haploid strains

作　　者：易弋[1] 赵东玲[1] 黎娅[1] 龚熠[1] 黄翠姬[1] 伍时华[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《中国酿造》2011年第11期42-46,共5页China Brewing

基　　金：广西科技厅项目(桂科攻0782003-2)资助;广西青年科学基金项目(桂科青0991010)资助

摘　　要：对酒精高产酿酒酵母GGFS16和GJ2008单倍体原生质体的形成与再生条件进行了研究。结果表明,在菌龄8h、0.2%β-巯基乙醇和0.1%EDTA-Na2预处理20min、1.5%的蜗牛酶37℃处理30min以及使用浓度170g/L的蔗糖作为渗透压稳定剂的条件下,GGFS16和GJ2008单倍体原生质体的形成率和再生率均达到最大。形成率分别是98.14%和97.19%,再生率分别是17.29%和15.78%。The conditions of formation and regeneration of protoplast from two high-alcohol-yield Saccharomyces cerevisiae haploid strains GGFS l 6 and GJ2008 were optimized. The optimum conditions were as follows： the haploid cultured for 8h, hydrolyzed with 1.5% snailase at 37℃ for 30 rain after pretreatment by 0.2% mercaptoethanol and 0.1% EDTA-Na2; 170g/L sucrose were used as osmotic stabilizer. Under these conditions, the formation rate of protoplast from the GGFS 16 and G J2008 haploid strains reached 98.14% and 97.19%, respectively. The regeneration rate reached 17.29% and 15.78%, respectively..

关 键 词：酿酒酵母 酒精高产 原生质体 形成率:再生率 

分 类 号：TS261.1[轻工技术与工程—发酵工程]