酶解丝素蛋白制备ACE抑制肽的研究  

Enzymatic Hydrolysis of Silk Fibroin for Production of ACE Inhibitors

作　　者：吴晖[1] 罗美琪[1] 唐语谦[1] 肖性龙[1] 袁坤[1] 李晓凤[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510561

出　　处：《现代食品科技》2011年第12期1461-1465,共5页Modern Food Science and Technology

基　　金：国家自然科学基金(20906031);新世纪优秀人才支持计划项目(NCET-060746);高校博士点基金项目(200805611022)

摘　　要：本研究首次探讨利用Alcalase酶解丝素蛋白制备高活性ACE抑制肽。以水解度为主要评价指标,研究了温度、浓度、pH、加酶量以及反应时间对酶解反应的影响。分析了不同水解度下的酶解产物与ACE体外抑制活性、肽得率和蛋白回收率的关系。确定了制备ACE抑制肽的最佳酶解反应DH值为17.12%,此时所得丝素蛋白肽的肽得率为50.31%,蛋白回收率为77.37%,ACE抑制率可达74.07%。基质辅助激光解吸电离-飞行时间质谱仪(MALDI-TOF)分析表明,所制备的丝素蛋白肽的分子量小于2.4 kDa。SiLk fibroin was hydrolyzed by Alcalase to produce the peptide with high ACE-inhibition activity. The effects of several influential factors on the enzymatic hydrolysis were investigated and the optimum reaction conditions were determined as follows： pH 8.5, temperature 60℃, substrate concentration 5%, enzyme dosage 1000 U/g protein, and reaction time 240 min, under which the DH, ACE-inhibition rate, peptide yield and protein recovery of the product were 17.12%, 74.07%, 50.31% and 77.37%, respectively. MALDI-TOF mass speetrometry of the hydrolysate showed that the molecular weight of the peptides was below 2.4kDa.

关 键 词：丝素蛋白 ACE抑制活性 活性肽 

分 类 号：TS201.2[轻工技术与工程—食品科学]