羊栖菜乙醇提取物的抗氧化活性  

Antioxidant Activities in Vitro of Ethanol Extract from Sargassum fusiforme

作　　者：叶红[1] 周春宏[2] 高蓓蕾[2] 王晓晴[1] 曾晓雄[1] 杨效华[1] 任德康[1] 

机构地区：[1]南京农业大学食品科技学院,南京210095 [2]江苏省环境监测中心,南京210036

出　　处：《湖北农业科学》2011年第22期4695-4697,共3页Hubei Agricultural Sciences

基　　金：南京农业大学青年科技创新基金(Y200415)

摘　　要：用体积分数70%的乙醇浸提羊栖菜(Sargassum fusiforme)得到粗提物,将粗提物溶于去离子水再依次用有机溶剂萃取,得到氯仿萃取物、乙酸乙酯萃取物、正丁醇萃取物和萃取后剩余物,测定粗提物和各萃取物的总多酚含量、维生素C和维生素E含量以及还原力。结果表明,正丁醇萃取物中的总多酚和维生素E含量较高,正丁醇萃取物和乙酸乙酯萃取物具有较强的还原力。Crude extract（CE） of Sargassum fusiforme was obtained using 70% ethanol as extracting solvent,dissolved in distilled water and then fractionated by inorganic solution including chloroform（Cf）,ethyl acetate（EtOAc）,and n-butanol（n-BuOH） orderly;Four fractions of Cf,EtOAc,n-BuOH and aqueous were collected.The antioxidant activities and contents of total polyphenols,vitamin C and E in CE and the four fractions were determined.Results showed that contents of total polyphenols and vitamin E were higher in n-BuOH fraction;While n-BuOH fraction and EtOAc fraction exhibited higher antioxidant activity.

关 键 词：羊栖菜(Sargassum fusiforme) 抗氧化活性 总多酚 DPPH. 

分 类 号：Q949.288.5[生物学—植物学] R284.2[医药卫生—中药学]