生乳细菌总数检测仪测定生乳细菌总数的应用  

Application of total bacteria measuring apparatus for detecting total bacteria in raw milk

作　　者：富鑫[1] 田雨[1] 管勇佳[1] 房乔[1] 李湘怡[1] 田波[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2012年第2期70-72,88,共4页Science and Technology of Food Industry

基　　金：东北农业大学科学研究启动基金;“十一五”国家科技支撑计划(2006BAD04A0814)

摘　　要：以还原法为理论基础,研制生乳细菌总数检测仪进行生乳细菌总数快速检测,以克服经典还原法目测比色主观性强的缺点,而且检测结果可以定量表示。该仪器连续检测加入试剂后的生乳样品颜色(RGB),通过数理统计分析,采用斜率法以R值斜率表示样品颜色的变化速度。研究以标准平板计数法(SPC法)为标准,建立了R值斜率与菌落总数之间的标准方程,经验证生乳细菌总数检测仪检测结果与SPC法检测结果差异不显著。与流式细胞仪比较,在与SPC法的相关性、运行成本及便携性等方面具有一定优势。A total bacteria measuring apparatus was developed for detecting total bacteria in raw milk, which overcame the defects of traditional reduction test measured by eyes,and might make a quantitative expression of total bacteria.The apparatus detected continuously the color （RGB） of the milk samples which had been added reagents,and the rate of color change was showed as R slope by statistical analysis. A standard curve was established to show the relation between R slope and total plate count.There was no significant difference between the two results of total bacteria measuring apparatus and standard plate count method. Comparing with flow cytometry,the total bacteria measuring apparatus had some advantages about better relativity with SPC, lower operating cost and more efficiency.

关 键 词：生乳细菌总数检测仪 还原法 细菌总数 生乳 

分 类 号：TS207.4[轻工技术与工程—食品科学]